Truffle powders (0.5 g) were submitted to pressurized liquid extraction using an Accelerated Solvent Extractor (ASE) (Dionex Corporation, ASE 350, USA) [6 (link)]. Briefly, samples were submitted to 16.7 MPa at 180 °C for 30 min. Fractions obtained with water were immediately frozen and freeze-dried in a LyoBeta 15 lyophilizer (Telstar, Madrid, Spain), and those obtained with ethanol were dried using a rotary vacuum evaporator at 40 °C (IKA® RV 10, VWR International, Barcelona, Spain). The water:ethanol extracts were dried and then lyophilized. Afterwards, samples were stored in darkness at −20 °C.
Lyobeta 15
The LyoBeta 15 is a laboratory freeze-dryer designed for efficient lyophilization of samples. It features a temperature-controlled chamber and a high-performance refrigeration system to facilitate the freeze-drying process.
Lab products found in correlation
14 protocols using lyobeta 15
Optimized Pressurized Liquid Extraction of Truffles
Truffle powders (0.5 g) were submitted to pressurized liquid extraction using an Accelerated Solvent Extractor (ASE) (Dionex Corporation, ASE 350, USA) [6 (link)]. Briefly, samples were submitted to 16.7 MPa at 180 °C for 30 min. Fractions obtained with water were immediately frozen and freeze-dried in a LyoBeta 15 lyophilizer (Telstar, Madrid, Spain), and those obtained with ethanol were dried using a rotary vacuum evaporator at 40 °C (IKA® RV 10, VWR International, Barcelona, Spain). The water:ethanol extracts were dried and then lyophilized. Afterwards, samples were stored in darkness at −20 °C.
Truffle Harvesting and Preparation
Sage Extract Production Protocol
Protein-rGO Binding Analysis by Spectroscopy
Raman spectrum was obtained by confocal Raman imaging (Alpha 300 M, Company WITec, Ulm, Germany) with a 532 nm laser (5% laser power, a contact time of the 50 s, and four accumulations). FT-IR, using an attenuated total reflectance (ATR) technique, was performed in a FT-IR Bruker IFS 66/S Spectrometer, with 32 scans at a resolution of 4 cm−1 between the wavelength ranges of 4000–400 cm−1. Air background was applied as a blank. At least three samples were analyzed for each condition.
Preparation of Chitosan Particles with Marjoram Extract
Fourier Transform Infrared Spectroscopy Analysis of GM-SLN
PLGA Nanoparticles for Melanoma Antigen Delivery
PEI-coated PLGA NPs were prepared using the solvent extraction–evaporation double emulsion (w/o/w) method, with encapsulation of melanoma patients’ antigens. Briefly, 50 mg of PLGA and 0.65 mg of PEI were dissolved in 1 mL of dichloromethane (DCM) and emulsified with a 1.25% (w/v) peptide solution in acetonitrile (ACN):water (50:50) by sonication (30 s using Branson sonifier 250). This emulsion was then mixed with 5 mL of 5% (w/v) PVA and sonicated for 1 min. The final emulsion was poured into a 2% (v/v) isopropanol solution (20 mL) and stirred for 2 h to allow solvent evaporation. Finally, the resulting nanoparticles were freeze-dried for 42 h with trehalose used as a cryoprotectant (Lyobeta 15, Telstar, Barcelona, Spain).
Insect Preparation for Animal Feed
Peptide-Loaded PLGA Nanoparticles Synthesis
Extraction and Characterization of Rosemary Leaf Extract
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