Tissue sections from all groups were fixed in 4% PFA at room temperature for one hour. They were then washed with PBS, mounted on glass and incubated in a solution of 10% normal goat serum in PBS (MP Biomedical, UK) and 0.25% Triton X-100 (Sigma, Germany) for one hour. Next, the tissues were incubated with 100 µL of a primary antibody (anti-MBP, MBL, anti-NG2 and anti-OX-42; Millipore, Germany) at room temperature for 90 min or in the dark at 4 °C overnight. They were washed three times with PBS and stained with secondary antibodies (Alexa Fluor® 633 and 488, Invitrogen, Germany) that were diluted in PBS (1:100). Of note, a few tissue sections were processed without primary antibodies in order to examine the specificity of immunolabelling with the antibodies, and this resulted in no immunostaining.
Anti ox42
Manufactured by Merck Group
Sourced in United States
Anti-OX42 is a laboratory reagent used to detect and study the expression of the CD11b/CD18 integrin, also known as the complement receptor 3 (CR3) or Mac-1, on various cell types. It is a monoclonal antibody that specifically binds to the OX-42 antigen present on the surface of myeloid cells, including macrophages, monocytes, and granulocytes.
Automatically generated - may contain errors
Lab products found in correlation
Inverted microscope, by Olympus (2 mentions)
Fluorescence microscope, by Olympus (2 mentions)
Anti gfap, by Merck Group (2 mentions)
Manganese chloride, by Merck Group (2 mentions)
Analytical pure lead acetate, by Merck Group (2 mentions)
Anti glast, by Merck Group (2 mentions)
Enzyme linked immunosorbent spectrophotometer, by Shimadzu (2 mentions)
Anti glt 1, by Merck Group (2 mentions)
Triton x 100, by Merck Group (1 mentions)
Anti mbp, by Merck Group (1 mentions)
Anti ng2, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by MP Biomedicals (1 mentions)
Alexa fluor 633 and 488, by Thermo Fisher Scientific (1 mentions)
Minocycline, by Merck Group (1 mentions)
Minocycline mc, by Merck Group (1 mentions)
3 protocols using anti ox42
1
Immunofluorescence Labeling of Tissue Sections
2
Analytical Techniques in Neuroscience
3
Immunohistochemical Analysis of Neurotransmitter Receptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analytical pure lead acetate and manganese chloride were purchased from SIGMA (USA). Anti-OX42, Anti-GFAP, Anti-GLAST, Anti-GLT-1 and Anti-GS antibodies were purchased from Millipore (Millipore, USA). Minocycline (MC) was obtained from Sigma (USA). All other reagents were purchased from Sigma (USA). The inverted microscope and the fluorescence microscope were purchased from Olympus (Tokyo, Japan). The Patch clamp system was bought from Axon (USA). The enzyme linked immunosorbent spectrophotometer was obtained from Shimadzu (Nakagyo-ku, Kyoto, Japan).
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