Anti bcl 2 rabbit mab

Primary HPMECs were obtained from ScienCell Research Laboratories and were maintained in ScienCell Endothelial Cell Medium in a humidified incubator at 37°C with 5% CO₂. LPS (from Escherichia coli 055:B5) and cycloheximide (CHX) were purchased from Sigma-Aldrich (St Louis, MO). The anti-YAP rabbit monoclonal antibody and the anti-YAP (phospho-Y357) rabbit polyclonal antibody were obtained from ABCAM. The anti-BAX rabbit mAb, anti-BCL-2 rabbit mAb, anti-P73 mouse mAb, anti-cleaved caspase-3 rabbit mAb, anti-cleaved-PARP rabbit mAb and anti-rabbit immunoglobulin-G-HRP-linked antibody were purchased from Cell Signaling Technologies (Danvers, Mass). The anti-mouse immunoglobulin-G-HRP-linked antibody, anti-pro-caspase-3 mouse mAb, anti-PARP mouse mAb and P73 siRNA (h):(sc-36167) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Control siRNA and YAP siRNA were synthesized by GenePharma Co, Ltd. (Shanghai, China). Alexa Fluor 568-labeled donkey anti-rabbit secondary antibody, Pro-Long Gold Antifade Mountant with DAPI and Lipofectamine RNA-iMAX were obtained from Invitrogen Life Science. The Annexin-V-FITC apoptosis kit was purchased from BD Biosciences.

Primary mouse pulmonary micro‐vascular endothelial cells (MPMECs) were obtained Angio‐Proteomie and maintained in Angio‐Proteomie Endothelial Cell Medium in a humidified 37°C, 5% CO₂ incubator. GSS was obtained from Shanghai Tian Xi Chemical Co., Ltd., and BAY11‐7082 was purchased from Beyotime of China. The LPS was obtained from Sigma‐Aldrich. Anti‐GEF‐H1 rabbit monoclonal antibody (mAb), anti‐Myd88 rabbit mAb, anti‐NF‐κB p65 rabbit mAb, anti‐rabbit phospho‐NF‐κB‐p65 rabbit antibody, anti‐BCL‐2 rabbit mAb and anti‐PARP rabbit mAb were obtained from Cell Signaling Technology. Anti‐CD31 antibody and anti‐cleaved‐PARP antibody were purchased from Santa Cruz Biotechnology. The Alexa Fluor 555‐conjugated goat antimouse secondary antibody and Alexa Fluor 594‐conjugated goat anti‐rabbit secondary antibody and the ProLong Gold Antifade Mountant with DAPI were obtained from Invitrogen Life Sciences.