Rabbit anti sycp3 ab15093

The following commercial antibodies were used: goat anti-DMC1 (sc-8973, C-20), goat anti-ATR (sc-1887, N19), mouse anti-SYCP3 (sc-74569, d-1), rat anti-GATA1 (sc-265) antibodies from Santa Cruz. Rabbit anti-H3K4me3 (07–473), from Millipore, rabbit anti-SYCP3 (ab15093) from Abcam, rabbit anti-γH2AX from Trevigen (4411-PC-100) and goat anti-PLZF (AF2944) from R&D systems. Secondary HRP antibodies were purchased from Jackson Laboratories. Fluorescent secondary Alexa antibodies were purchased from Invitrogen.

Rabbit anti-SIRT6 polyclonal antibody was purchased from sigma (S4197) for western blotting and Abcam(ab62739) for immunofluorescence staining. Mouse anti-SYCP3 (150292) and rabbit anti-SYCP3 (ab15093) were purchased from Abcam (Cambridge, MA). Rabbit anti-SYCP1 (NB300-228c) was purchased from Novus Biologicals (Littleton, CO). Mouse anti-γH2AX (05-636) was purchased from Merck Millipore (Darmstadt, Germany). Rabbit anti-H2A (ab18975) was purchased from Abcam (Cambridge, MA). Rabbit anti-H3K9ac(P30050M) and mouse anti-ACTIN antibody (M20011L) were purchased from Abmart, H3K18ac and H3K56ac antibody kits (9927T) were purchased from Cell Signaling Technology. Alexa Fluor 488 -lectin PNA was purchased from Molecular Probes. Goat FITC-conjugated secondary antibodies for rabbit, goat FITC-conjugated secondary antibodies for mouse, goat TRITC conjugated secondary antibodies for rabbit, and goat TRITC conjugated secondary antibodies for mouse were purchased from Zhong Shan Jin Qiao (Beijing, China). Alexa Fluor 680-conjugated goat anti-mouse and Alexa Fluor 800-conjugated goat anti-rabbit secondary antibodies, used in western blot analysis, were purchased from Invitrogen.