1H NMR spectra were obtained using a Bruker 300 MHz spectrometer. UV-Visible spectra were obtained using an Agilent 8453 spectrometer equipped with a photodiode array detector. Fluorescence spectra were obtained using a Shimadzu RF-5301PC spectrophotofluorimeter. All GCMS measurements were obtained using a Shimadzu GCMS-QP2020 gas chromatograph-mass spectrometer. All toxicants and toxicant metabolites (compounds 1–10 , Figure 1 ) were purchased from Sigma Aldrich and used as received. Fluorophore 11 was synthesized following literature-reported procedures.46 (link) Fluorophores 12 and 13 were purchased from Sigma Aldrich and used as received.
Rf 5301pc spectrophotofluorimeter
Manufactured by Shimadzu
The RF-5301PC is a spectrophotofluorimeter by Shimadzu. It is a compact and versatile fluorescence spectrophotometer designed for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
300 mhz spectrometer, by Bruker (3 mentions)
8453 spectrometer, by Agilent Technologies (2 mentions)
Gcms qp2020 gas chromatograph mass spectrometer, by Shimadzu (1 mentions)
Uv 3600 plus spectrophotometer, by Shimadzu (1 mentions)
Orion 3 star benchtop conductivity kit, by Thermo Fisher Scientific (1 mentions)
4 protocols using rf 5301pc spectrophotofluorimeter
1
Comprehensive Analytical Characterization of Toxicant Metabolites
2
Organochlorine Pesticide Analysis Protocols
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All organochlorine
pesticides and control analytes (compounds1–5 , Figure 1 ) were purchased
from Sigma-Aldrich chemical company and used
as received, unless otherwise noted. Fluorophore6 was
synthesized following literature-reported procedures.49 (link)1H NMR spectra were recorded using a Bruker
300 MHz spectrometer. UV–visible spectra were recorded using
a Shimadzu UV-3600 Plus spectrophotometer. Fluorescence spectra were
recorded using a Shimadzu RF-5301PC spectrophotofluorimeter. The following
commercially available cyclodextrin derivatives were used as received:
α-cyclodextrin, β-cyclodextrin, randomly methylated β-cyclodextrin,50 (link) 2-hydroxypropyl β-cyclodextrin, and γ-cyclodextrin.
For the temperature-dependent studies, a Fisher Scientific Isotemp
6200 R20 instrument was used to control the temperature, and the spectrophotometer
was equipped with a single constant-temperature cell holder. All gas
chromatography–mass spectrometry (GC–MS) measurements
were obtained using a Shimadzu GC–MS-QP2020 gas chromatograph–mass
spectrometer. Conductivity measurements were obtained using a Thermo
Scientific Orion 3-Star Benchtop Conductivity Kit. All pH experiments
were performed using a MicroLab FS-522 instrument.
pesticides and control analytes (compounds
from Sigma-Aldrich chemical company and used
as received, unless otherwise noted. Fluorophore
synthesized following literature-reported procedures.49 (link)1H NMR spectra were recorded using a Bruker
300 MHz spectrometer. UV–visible spectra were recorded using
a Shimadzu UV-3600 Plus spectrophotometer. Fluorescence spectra were
recorded using a Shimadzu RF-5301PC spectrophotofluorimeter. The following
commercially available cyclodextrin derivatives were used as received:
α-cyclodextrin, β-cyclodextrin, randomly methylated β-cyclodextrin,50 (link) 2-hydroxypropyl β-cyclodextrin, and γ-cyclodextrin.
For the temperature-dependent studies, a Fisher Scientific Isotemp
6200 R20 instrument was used to control the temperature, and the spectrophotometer
was equipped with a single constant-temperature cell holder. All gas
chromatography–mass spectrometry (GC–MS) measurements
were obtained using a Shimadzu GC–MS-QP2020 gas chromatograph–mass
spectrometer. Conductivity measurements were obtained using a Thermo
Scientific Orion 3-Star Benchtop Conductivity Kit. All pH experiments
were performed using a MicroLab FS-522 instrument.
3
Synthesis and Characterization of Compound 10
4
Fluorescence Spectroscopy of Cyclodextrin Complexes
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Fluorescence measurements were
recorded on a Shimadzu RF-5301PC spectrophotofluorimeter with 1.5
nm excitation and 1.5 nm emission slit widths. All analytes and fluorophores
(compounds1 –17 , Figure 2 ) were purchased from Sigma-Aldrich
Chemical Company and used as received. All cyclodextrins were purchased
from Tokyo Chemical Industry and used as received. Computational experiments
were performed using the Spartan 18 software for electrostatic potential
maps and Molecular Operating Environment for system modeling. All
GC–MS measurements were performed using a Shimadzu GC–MS
QP-2020 gas chromatograph–mass spectrometer.
recorded on a Shimadzu RF-5301PC spectrophotofluorimeter with 1.5
nm excitation and 1.5 nm emission slit widths. All analytes and fluorophores
(compounds
Chemical Company and used as received. All cyclodextrins were purchased
from Tokyo Chemical Industry and used as received. Computational experiments
were performed using the Spartan 18 software for electrostatic potential
maps and Molecular Operating Environment for system modeling. All
GC–MS measurements were performed using a Shimadzu GC–MS
QP-2020 gas chromatograph–mass spectrometer.
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