Las 3000 imager device

Manufactured by Fujifilm

The Fujifilm Las-3000 is an imager device designed for laboratory use. It captures and digitizes images from various types of gels and membranes. The device utilizes a CCD camera and advanced optics to produce high-quality digital images for further analysis and documentation.

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Lab products found in correlation

Protease inhibitor cocktail tablet, by Roche (2 mentions) Ecl plus reactive, by Thermo Fisher Scientific (2 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 phalloidin, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Skimmed milk, by Merck Group (1 mentions) Trans blot semi dry transfer cell, by Bio-Rad (1 mentions) Mini protean electrophoresis system, by Bio-Rad (1 mentions)

Close spelling variants of this product

LAS-3000 (605 citations) LAS-3000 imager (221 citations) LAS-3000 device (7 citations)

2 protocols using las 3000 imager device

Zip7 Expression in Cell Differentiation

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Distribution of Zip7 was analysed in non-differentiation (after 24 h) and differentiation (after 6 days) cell stages by immunofluorescence, using specific antibody against Zip7 (dil: 1/200, Santa Cruz Biotechnologies). Primary antibody was incubated over night at 4 °C. After washing, anti-goat Dye Light 488 (dil: 1/500, Thermofisher) secondary antibody was added and incubated for 1 h at room temperature. Cell cytoskeleton was labelled using Alexa Fluor 555 Phalloidin (dil: 1/100, Thermofisher) and cell nucleus with Hoechst. Cells were imaged by Nikon Eclipse i80 fluorescence microscope.
For protein expression analysis, total protein extraction was performed with RIPA buffer supplemented with protease inhibitor cocktail tablets (Roche). Proteins were separated in 12% SDS-PAGE as described previously^{40 (link)}. Primary antibodies against Zip7 (dil: 1/300, Santa Cruz Biotechnologies), Akt (dil: 1/1,000; Thermofisher), phospho Akt (pAkt-serine 473) (dil: 1/700; Thermofisher) and Glyceraldehyde 3-phosphate dehydrogenase (GapDH, dil: 1/5,000; Thermofisher) were incubated over night at 4 °C. Then, membranes were washed and incubated with HRP- linked secondary antibody for chemiluminiscence band detection with ECL-Plus reactive (Thermofisher). Fujifilm Las-3000 imager device was used for protein bands visualisation.

Mnatsakanyan H., Serra R.S., Rico P, & Salmerón-Sánchez M. (2018). Zinc uptake promotes myoblast differentiation via Zip7 transporter and activation of Akt signalling transduction pathway. Scientific Reports, 8, 13642.

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Western Blot Protein Detection

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Protein was extracted by RIPA buffer supplemented with protease inhibitor cocktail tablets (Roche). The proteins were separated in 10% SDS-PAGE with Mini-PROTEAN Electrophoresis System (Bio-Rad) and transferred to PVDF (GE-Healthcare) membranes by Trans-Blot Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked with 5% BSA (Sigma-Aldrich) or 5% skimmed milk (Sigma-Aldrich), in TBS/0.1% Tween 20 (Sigma-Aldrich) for 1 h and incubated with primary antibodies (Table S2) diluted in TBS, 0.1% Tween 20 and 3% BSA overnight at 4° C. Membranes were washed and incubated with HRP-linked secondary antibody (Table S2) for 1 h at RT.
The chemiluminescence band was detected by ECL-Plus reactive (ThermoFisher). A Fujifilm Las-3000 imager device was used to visualize the protein bands.

Mnatsakanyan H., Salmerón‐Sánchez M., & Rico P. (2020). Lithium directs embryonic stem cell differentiation versus hemogenic endothelium.

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