Sucrose (S7903), D -glucose (G7528), L -glucose (G5500), D -arabinose (10850), denatonium benzoate (D5765) and 2-Deoxy-D -glucose (D -6134) were purchased from Sigma-Aldrich. Sucralose (65684B) and phlorizin (69592A) were purchased from Adamas. Trehalose (K480), sorbitol (0691), D -fructose (0226) and D -ribose (0671) were purchased from AMRESCO. Yeast extract (LP0021) and tryptone (LP0042) were from OXOID. Quinine (Q0030) was purchased from TCI AMERICA.
Tryptone lp0042
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Tryptone LP0042 is a powdered nutritional supplement used as a component in culture media formulations for the cultivation of microorganisms. It is composed primarily of peptides derived from the enzymatic digestion of casein.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract lp0021, by Thermo Fisher Scientific (2 mentions)
Denatonium benzoate, by Merck Group (1 mentions)
D arabinose, by Merck Group (1 mentions)
2 deoxy d glucose, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail, by Merck Group (1 mentions)
Anti mouse pd 1 antibody clone rmp1 14, by BioXCell (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphate dopa, by Avanti Polar Lipids (1 mentions)
2 3 dioleoyloxy propyl trimethylammonium dotap, by Avanti Polar Lipids (1 mentions)
Gemcitabine, by Eli Lilly (1 mentions)
Casein hydrolysate, by Merck Group (1 mentions)
Primerscript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)
Trizol, by Sangon (1 mentions)
4 protocols using tryptone lp0042
1
Carbohydrate Characterization and Sourcing
2
Formulation and Characterization of DOTAP/DOPA Lipoplex
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(2,3-dioleoyloxy-propyl)-trimethylammonium (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) was obtained from Xi’an Ruixi biotech (Xi’an, China). FHK peptide (FHKHKSPALSPV) was synthesized by China Peptides Co., Ltd (Shanghai, China). Low molecular weight protamine (LMWP), IGEPAL® CO-520, cholesterol, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), 4,6-Diamidino-2-phenylindole (DAPI), Hoechst 33258 and phosphatase inhibitor cocktails were provided by Sigma-Aldrich (St. Louis, MO, USA). α-M were purchased from Biopurify Co., Ltd (Chengdu, China). Collagenase type IV (40510ES60), hyaluronidase (20426ES60), DNase I (10608ES25) and Agar (70101ES76) were purchased from Shanghai yeasen biotech (Shanghai, China). Tryptone (LP0042) and Yeast extract (LP0021) were obtained from OXOID. Gemcitabine was obtained from Eli Lilly. Anti-mouse PD-1 antibody (clone: RMP1-14) and IgG isotype (MPC-11, BP0086) are acquired from BioXcell. All the other chemical reagents and solvents were acquired from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) unless specified.
3
Caffeate Derivatives Characterization Protocol
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Tryptone LP0042 was purchased from Oxoid, UK. Casein hydrolysate, PLGA (lactide : glycolide = 50 : 50; intrinsic viscosity: 0.45–0.60 dL g−1; Mw: ∼38–54 kDa P50/50), and PVA 124 (viscosity: 40 g L−1, 20 °C, 54.0–66.0 (mPa s)−1) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Caffeate derivatives such as MC and CAPE were prepared by our group.21,23 (link) SYBR premix Ex Tap™ II (Tli RNaseH Plus), primer script RT reagent kit with gDNA eraser (Perfect Real Time), DEPC water, and isopropanol were purchased from Takara. The total RNA extraction reagent TRIzol and PCR kit were purchased from Sangon Biotech. RS-5 R. solanacearum strains were used, which were isolated from mulberry roots.21 (link) All the primers were synthesized by Shanghai Biotech; the primer sequences were synthesized as described elsewhere,18 (link) as listed in Table 1 .
4
Molecular Characterization of R. solanacearum
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The R. solanacearum strain (RS-5) was isolated from diseased mulberry tree leaves and identified with gram staining and a serological response [35] (link). Antibodies against R. solanacearum strains (Prajna Biology, Shanghai, China), polylactic-co-glycolic acid (PLGA) and polyvinyl alcohol (PVA) 124 (St. Louis, Missouri, American, analytically pure reagent), Tryptone LP0042 (Oxoid, Basingstoke, UK, analytically pure reagent), hydrolyzed casein and methyl caffeate (research group), and phenethyl caffeate (Macleans, Hong Kong, China) [36, (link)37] (link). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) (Sinopharm, Shanghai, China, analytically pure reagent). Ltd. DEPC water and isopropanol (Takara). The total RNA extraction reagent TRIzol and PCR kit (Sangon, Shanghai, China). All of the primers were purchased from Shanghai Sangon Biotech. A Roche LightCycler96 real-time fluorescent quantitative PCR instrument (Roche, Shanghai, China).
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