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Abx micros esv 60

Manufactured by Horiba
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Sourced in Japan, France

The ABX Micros ESV 60 is a compact, fully automated hematology analyzer designed for routine clinical laboratory use. It performs complete blood count (CBC) analysis, including red blood cell (RBC), white blood cell (WBC), and platelet (PLT) counts, as well as hemoglobin (HGB) and hematocrit (HCT) measurements. The device is capable of processing up to 60 samples per hour and requires a small sample volume for analysis.

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Lab products found in correlation

Vacutainer, by BD (2 mentions) Ketamine hydrochloride, by Syntec (1 mentions) Rx daytona, by Randox (1 mentions)

Spelling variants of this product

ABX Micros 60 (56 citations) Micros 60 (14 citations)

10 protocols using abx micros esv 60

1

Blood Cell Analysis in Mice

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The day before the beginning of Epo or saline administration and at the end of the time of each treatment (30 dpi or 120 dpi) the packed cell volume or hematocrit (Hct) was measured. For that, mice were i.p. anesthetized with ketamine hydrochloride 10% and xylazine hydrochloride 2% based on their body weight (2:1; maximal dose 10 mL/kg) (Syntec) and blood from orbital sinus was collected in heparinized capillary microtubes. Hct was obtained by the microhematocrit method using a microcentrifuge (MH 11.5i, Inbras).
At baseline (d0) and immediately before the euthanasia (d180) blood samples were collected as described above. Complete blood count (CBC) was performed and hemoglobin concentration (HGB) was determined using an automatic cell counter (ABX® Micros ESV 60, Horiba). Hct was determined by the microhematocrit method. Whole blood smears were prepared and stained with Diff-Quick (Instant Prov, Newprov, Brazil) for leukocyte differential count. The smears were analyzed by light microscopy and at least 100 white blood cells (WBC) were counted.
de Castro Nobre A.C., Pimentel C.F., do Rêgo G.M., Paludo G.R., Pereira Neto G.B., de Castro M.B., Nitz N., Hecht M., Dallago B, & Hagström L. (2022). Insights from the use of erythropoietin in experimental Chagas disease. International Journal for Parasitology: Drugs and Drug Resistance, 19, 65-80.
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2

Comprehensive Biomarker Analysis in Animal Study

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At the end of the study, blood samples were collected for haematology, clinical pathology investigations and biomarker analysis. The haematology analyzer (model ABX Micros ESV 60; HORIBA Instruments Inc., Irvine, CA, USA) was used for haematological evaluation. Clinical chemistry parameters were estimated using the RX Daytona+ (Randox Laboratories, Kearneysville, WV, USA) instrument. Commercially available enzyme-linked immunosorbent assay kits (KinesisDx kits; Krishgen Biosystems, Mumbai, India) were used to quantify insulin, leptin, adiponectin and free fatty acids. At the end of the study, all animals were subjected to a detailed gross necropsy examination. External body surfaces, orifices and cavities such as abdominal, thoracic and cranial were included in the examination.
Shanmugham V, & Subban R. (2022). Comparison of the Anti-Obesity Effect of Enriched Capsanthin and Capsaicin from Capsicum annuum L. Fruit in Obesity-Induced C57BL/6J Mouse Model. Food Technology and Biotechnology, 60(2), 202-212.
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3

Hematological Evaluation of Pigs

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On day 49 of age, eight pigs from each treatment group were randomly selected and 5 mL of blood was collected from their jugular veins with 21 G needles (Covidien, Mansfield, MA, USA) (1.5 inches) under sterile conditions. Blood plasma was collected in Vacutainer tubes with lilac caps (BD Vacutainer; BD, Franklin Lakes, NJ, USA) containing an anticoagulant agent (200 µL sodium heparin). Blood serum was collected in Vacutainer tubes (with red caps) without an anticoagulant agent and was centrifuged at 3000× g for 15 min (Eppendorf centrifuge AG, New York, NY, USA) at room temperature.
The following hematological variables were evaluated in a semi-automatic analyzer (Horiba ABX Micros ESV 60®; Paris, France): White blood cells (WBC); red blood cells (RBC); hemoglobin; hematocrit; mean corpuscular volume (MCV); mean corpuscular hemoglobin concentration (MCHC); mean cell volume (MCV); mean platelet volume. All samples were processed in the Veterinary Clinical Laboratory of the School of Veterinary Medicine and Zootechnics of the University of Córdoba, Colombia.
Betancur C., Martínez Y., Merino-Guzman R., Hernandez-Velasco X., Castillo R., Rodríguez R, & Tellez-Isaias G. (2020). Evaluation of Oral Administration of Lactobacillus plantarum CAM6 Strain as an Alternative to Antibiotics in Weaned Pigs. Animals : an Open Access Journal from MDPI, 10(7), 1218.
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4

Plasma Sampling and CBC Analysis

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Blood samples (125 µL) were taken from the retroorbital plexus (blank sample) after the drug administration at several time points (5 min, 15 min, 30 min, 60 min, 6 h 12 h, 48 h and 72 h) in tubes containing EDTA as an anticoagulant. Each blood sample was gently inverted several times to ensure complete mixing with the anticoagulant. After centrifugation at 5000 × g for 10 min, plasma samples were separated and stored at -20 °C until analysis. CBC were analyzed using ABX Micros ESV60 (Horiba).
Zaleskis G., Garberytė S., Pavliukevičienė B., Valinčius G., Characiejus D., Mauricas M., Kraśko J.A., Žilionytė K., Žvirblė M, & Pašukonienė V. (2020). Doxorubicin uptake in ascitic lymphoma model: resistance or curability is governed by tumor cell density and prolonged drug retention. Journal of Cancer, 11(22), 6497-6506.
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5

Hematological Analysis of Blood Samples

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The blood was collected in tubes containing 7.0% EDTA and the analysis of hematological parameters was performed in an ABX Micros ESV60 (Horiba, Kyoto, Japan) cell counter by the Veterinary Hospital of Brasilia University. The following parameters were analyzed: erythrocytes, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (HCM), mean corpuscular hemoglobin concentration (MCHC), platelets, and white blood cells.
Campos N.A., da Cunha M.S, & Arruda S.F. (2018). Tucum-do-cerrado (Bactris setosa Mart.) modulates oxidative stress, inflammation, and apoptosis-related proteins in rats treated with azoxymethane. PLoS ONE, 13(11), e0206670.
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6

Automated Hematological Analysis of EDTA Blood Samples

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Complete blood counts (CBCs) were performed on 90 out of the 100 EDTA-anticoagulated blood samples within 24 h of blood collection using an automated haematological cell counter ABX Micros ESV 60 (Horiba, Kyoto, Japan). The parameters evaluated in the hemogram included haematocrit (HCT), haemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total white blood cell counts (WBCs), and total platelet counts (PLTs).
Díaz-Sánchez A.A., Corona-González B., Meli M.L., Roblejo-Arias L., Fonseca-Rodríguez O., Pérez Castillo A., Vega Cañizares E., Lobo Rivero E, & Hofmann-Lehmann R. (2020). Molecular Diagnosis, Prevalence and Importance of Zoonotic Vector-Borne Pathogens in Cuban Shelter Dogs—A Preliminary Study. Pathogens, 9(11), 901.
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7

Hematological Profile Analysis in Blood Plasma

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In blood plasma, the following hematological variables were evaluated in a semiautomatic analyzer (Horiba ABX Micros ESV 60®; Paris, France): hemoglobin, hemogram, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (CMCH), mean corpuscular hemoglobin (MCH), and mean platelet volume (MPV). All the samples were processed in the Veterinary Clinical Laboratory of the Faculty of Veterinary Medicine and Zootechnics, University of Córdoba.
Betancur C., Martínez Y., Tellez-Isaias G., Castillo R, & Ding X. (2021). Effect of Oral Administration with Lactobacillus plantarum CAM6 Strain on Sows during Gestation-Lactation and the Derived Impact on Their Progeny Performance. Mediators of Inflammation, 2021, 6615960.
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8

Comprehensive Blood Analysis Protocol

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The blood samples were analyzed for total leukocyte count (TLC), differential leucocyte count (DLC), red blood cells (RBC), hemoglobin (Hb), packed cell volume (PCV), total platelet count (TPC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) using an automated blood analyzer (ABX Micros ESV60, Horiba, Kyoto, Japan).
Saminathan M., Singh K.P., Maity M., Vineetha S., Manjunathareddy G.B., Dhama K., Malik Y.S., Ramakrishnan M.A., Misri J, & Gupta V.K. (2021). Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice. Journal of Advanced Research, 31, 137-153.
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9

Rodent Hematological Profiling

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The hematological parameters: red blood cells (RBC), hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV), platelets (PTL), and white blood cells (WBC) were determined in a cell counter (ABX Micros ESV 60, Horiba, Kyoto, Japan) using 1.5 mL of the blood. The cell counter was calibrated and the parameters were defined for rats.
da Cunha M.D, & Arruda S.F. (2017). Tucum-do-Cerrado (Bactris setosa Mart.) May Promote Anti-Aging Effect by Upregulating SIRT1-Nrf2 Pathway and Attenuating Oxidative Stress and Inflammation. Nutrients, 9(11), 1243.
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10

Porcine Blood Parameter Analysis

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At the conclusion of the experiment, 12 pigs were randomly selected from each treatment group after fasting, and blood samples of 5 mL were collected through jugular vein puncture into heparinized Vacutainer tubes with lilac caps (BD Vacutainer; BD, Franklin Lakes, NJ, USA). The tubes, placed in containers with ice packs, were transported to the laboratory and kept at 4 °C until centrifugation at 1381× g (Eppendorf centrifuge AG, New York, NY, USA) for 15 min at room temperature.
The plasma samples were processed at the Veterinary Clinical Laboratory of the Faculty of Veterinary Medicine and Zootechnics, University of Córdoba. The samples were analyzed for white blood cells (WBC), red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean cell volume (MCV), and mean platelet volume (MPV) using a semiautomatic analyzer (Horiba ABX Micros ESV 60®; Paris, France).
Betancur C, & Martínez Y. (2023). Effect of Oral Administration with Lactobacillus plantarum CAM6 on the Hematological Profile, Relative Weight of Digestive Organs, and Cecal Traits in Growing Pigs. Animals : an Open Access Journal from MDPI, 13(12), 1915.
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