To synthesize the HA nanopowder co-precipitation technique is applied and to get this done, calcium chloride (CaCl2, Merck, Germany) and trisodium phosphate dodecahydrate (Na3PO4. 12H2O, Merck, Germany) are used. First, two separate solutions for CaCl2 and Na3PO4. 12H2O are provided and it should be mentioned that the chloride solution temperature is kept at 45°C, whereas the phosphate solution's temperature is set at 95°C. Second, the chloride solution is then added dropwise to the phosphate solution under continuous stirring. Finally, the precipitates are filtered, rinsed, and dried for 24 h followed by calcination at 900°C with 10°C min-1 heat rate for 3 h. It should be noticed that the molar ratio of Ca2 + to PO4 -3 is 1.67 to obtain HA nanopowder.
Na3po4 12h2o
Manufactured by Merck Group
Sourced in Germany
Na3PO4·12H2O is a chemical compound that is commonly used as a laboratory reagent. It is a crystalline solid with the chemical formula Na3PO4·12H2O, which represents sodium phosphate dodecahydrate. This compound is soluble in water and is often used in various chemical and analytical applications in research and testing environments.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Naclo2, by Merck Group (2 mentions)
Na2hpo4, by Merck Group (2 mentions)
Cacl2, by Merck Group (1 mentions)
K4fe cn 6, by Merck Group (1 mentions)
K3fe cn 6, by Merck Group (1 mentions)
Bsa protein, by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Aniline, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Curcumin powder, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Fecl3 6h2o, by Merck Group (1 mentions)
Na2hpo4 2h2o, by Honeywell (1 mentions)
Sodium phosphate dibasic dihydrate, by Honeywell (1 mentions)
Succinic anhydride, by Merck Group (1 mentions)
Hplc plus grade, by Merck Group (1 mentions)
Phosphoric acid h3po4, by Merck Group (1 mentions)
9 protocols using na3po4 12h2o
1
Synthesis of Hydroxyapatite Nanopowder
2
Protein Characterization of BSA
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BSA protein was obtained from Sigma-Aldrich Co. BSA solution (1 mg mL−1) was prepared by dissolving BSA in distilled water. KCl, Na2HPO4·5H2O, NaH2PO4, K3 [Fe(CN)6] and K4 [Fe(CN)6] (Merck Co.) used to prepare buffer and probe solutions. Aniline (Merck Co.) was purified by double vacuum distillation. All solvents, ammonium persulfate (APS), epichlorohydrin, sodium ethoxide, Tin (Sn), Na3PO4·12H2O, and hydrochloric acid (HCl) were purchased from Merck Company and used without further purification. l -lactic acid was supplied by Fluka Company.
3
Hydrothermal Synthesis of CeO2@WS2 Catalyst
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The detailed synthesis procedure of the CeO2@WS2 catalyst is reported in our previous work [15] (link). In brief, the WS2 nanoflakes were obtained by ultrasound-assisted exfoliation (Sonica ultrasound bath, 300 W, Italy) of bulk WS2 (Sigma-Aldrich, 99 %) in DMF (Merck, >99 %) for 2 h. After centrifuging the sample, the supernatant was removed, washed with EtOH, and dried at room temperature overnight. For synthesising CeO2@WS2, 0.1 g of the synthesised WS2 nanoflakes were added to 60 mL 0.1 mol/L Ce(NO3).6H2O (Merck, >99.99 %) in aqueous solution and sonicated for 3 h. After that, the mixture was added to a Na3PO4·12H2O (Merck, >99.99 %) solution (0.005 mol/L, 20 mL) and was stirred for 30 min. The final mixture was poured into a 100 mL hydrothermal autoclave and kept at 220 °C for 12 h. After cooling the autoclave, the liquid was removed by centrifuge and the remaining was washed with EtOH and deionised water and dried at 60 °C. The synthesis route for pure CeO2, used in this work, was similar to the mentioned approach without adding WS2 nanoflakes.
4
Spectrophotometric Quantification of Iron(III) with Curcumin
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The chemicals and solvents were of analytical grade, and deionized water was used for preparing the solutions; 0.400 g of Na3PO4•12H2O (Merck, Darmstadt, Germany) was dissolved in water and diluted to 100 mL to give a phosphate solution of 1000 μg mL -1 . Other concentrations were made daily from this solution by appropriate dilutions. An iron(III) solution of 1000 μg mL -1 was prepared as follows: 0.4840 g of the FeCl3•6H2O (Merck) and 1 mL of hydrochloric acid (Merck) was added to a 100-mL volumetric flask and diluted to the mark with water. For the preparation of acetate buffer (pH 4), 0.10 mol L -1 of NaOH (Merck) was added to 0.01 mol L -1 of acetic acid (Merck), and the pH was adjusted to 4 by a pH meter. Curcumin powder was purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Wood Functionalization using Succinic Anhydride
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Sapwood of spruce wood (Picea abies) from Switzerland was used. For wood functionalization, succinic anhydride and acetone were purchased from Sigma-Aldrich (St. Louis, MI, USA). Pyridine (anhydrous grade) was obtained from VWR (Radnor, PA, USA). Tip functionalization required 11-mercaptoundecanoic acid (HOOC(CH2)10SH, ≥ 95%, Sigma-Aldrich), 11-mercapto-1-undecanol (OH(CH2)11SH, ≥ 97%, Sigma-Aldrich) and ethanol (HPLC grade, ≥ 99.8%, Sigma-Aldrich). Buffer preparation in the pH range from pH 2 to pH 12 required water (HPLC plus grade, Sigma Aldrich), monobasic sodium phosphate (NaH2PO4 anhydrous, ≥ 99.0%, Sigma-Aldrich), phosphoric acid (H3PO4, 85wt%, Sigma-Aldrich), sodium phosphate dibasic dihydrate (Na2HPO4*2H2O, ≥ 99.5%, Riedel-de Haën, Seelze, Germany) and trisodium phosphate dodecahydrate (Na3PO4*12H2O, ≥ 98%, Sigma-Aldrich).
6
Copper Electroplating and Microfabrication
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PDMS (Sylgard 184), Cu electroplating kits, including Microfill EVF Carrier, Brightner, and Leveler, were purchased from Dow Corning, Midland, MI, USA. Potassium hydroxide pellet (KOH) was purchased from Fisher Scientific, Waltham, MA, USA. AZ 5214 photoresist was purchased from MicroChemicals, Ulm, Germany. Trichloro(1H,1H,2H,2H-perfluorooctly)silane, NaClO2, NaOH, and Na3PO4·12H2O were purchased from Sigma Aldrich, St. Louis, MI, USA. Cu etchant (CE-100) was purchased from Transene, Danvers, MA, USA. Hydrochloric acid (HCl) was purchased from Macron Fine Chemicals, Allentown, PA, USA.
7
Colorimetric Detection of Silver Ions
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All chemicals were used as received: silver nitrate (AgNO3, 99.8%, Penta industry; Prague, Czech Republic), methyl orange (MO, analytical reagent, Alfa Aesar; Tewksbury, MA, USA), Milli-Q (MQ; Budapest, Hungary) water, phosphoric acid (H3PO4, 85%, VWR Chemicals; Radnor, PA, USA), monosodium phosphate (NaH2PO4; >99.0%, Spektrum-3D; Debrecen, Hungary), disodium phosphate (Na2HPO4; >99.0%, Sigma-Aldrich; Schnelldorf, Germany), and trisodium phosphate dodecahydrate (Na3PO4·12H2O; analytical reagent, Sigma-Aldrich, Schnelldorf, Germany).
8
Nanostructured CuO/TiO2 Hybrid Meshes for Environmental Applications
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Pre-cleaned copper sheet and meshes (M120, M200, M300, and M500, pore size 110, 74, 46, and 32 μm, Anpin Keian Metal Meshes, Co. LTD) were immersed in aqueous solutions of NaClO2, NaOH, and Na3PO4·12H2O (Sigma Aldrich) with different mole ratios for 5 min at 90 °C. Details of chemical oxidation using various molar ratios of oxidants is given in supporting information . After chemical oxidation, the intrinsic reddish brown color of copper meshes turned to deep black. TiO2 was coated on nanostructured CuO sheet/mesh using various approaches, like sol gel layer by layer (LBL) assembly, hydrothermal, sputtering followed by hydrothermal method. As-deposited TiO2 nanostructures on Cu and CuO substrates using various approaches were named as TNS/Cu-m. Where, ‘m’ corresponds to “I, II, and III” for LBL assembly, sputtering followed by hydrothermal methods, and hydrothermal treatment, respectively. Thin film of TiO2 120 nm thickness was deposited on glass and Cu substrate using sputtering of 99.9% TiO2 target. The schematic of fabrication of TNS- Cu mesh is given in Fig. 1 .
9
Synthesis and Characterization of Calcium Phosphates
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Na3PO4·12H2O and Na2HPO4 were purchased from Sigma-Aldrich; methanol, ethanol, DCPD and CaCl2 were purchased from Sinopharm Chemical Reagent Co.; sodium polyacrylate (NaPAA, 50% solution, Mw 3000–5000) was purchased from Aladdin Reagent. All chemicals were used as received without further purification. All of the chemicals were of analytical grade. DCP was prepared by heating DCPD at 195 °C for 1 h and was confirmed by XRPD. Deionized water was used in all experiments.
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