100 nM of RFC:PCNA was applied on carbon-coated 400-mesh grids. Excess sample was blotted from the grid surface, the grids were washed twice with 50 mM HEPES, pH 7.5 and stained with 1% uranyl acetate. RFC:PCNA was imaged on a 120 kV Philips CM-120 microscope fitted with a Gatan Orius SC1000 detector.
Cm 120 microscope
Manufactured by Ametek
The CM-120 microscope is a laboratory equipment product designed for high-resolution imaging. It features a compact and robust construction, providing a stable platform for precise observations. The CM-120 utilizes advanced optical technology to deliver clear and detailed images, enabling users to analyze and inspect samples with precision.
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Lab products found in correlation
3 protocols using cm 120 microscope
1
Visualization of RFC:PCNA Complex
2
Thermus thermophilus Virus Imaging
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Infections of Thermus thermophilus HB8 were performed as described above (growth/purification of P74-26 virions). Aliquots of the infection culture were diluted 1:1 in Thermus growth medium and applied to carbon-coated 400-mesh grids 1 h after infection. Excess sample was then blotted from the grid surface, and grids were finally stained with 1% uranyl acetate. Data were then collected on a 120 kV Philips CM-120 microscope fitted with a Gatan Orius SC1000 detector.
3
Negative-Staining EM of hRFC
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Negative-Staining EM Purified hRFC was diluted to a concentration of 100 nM and applied on carbon-coated 400-mesh grids. After blotting, the grids were washed twice with 50 mM Hepes pH 7.5 and stained with 1% uranyl acetate. Data were collected on a 120 kV Philips CM-120 microscope fitted with a Gatan Orius SC1000 detector.
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