Cell viability was measured using a MTT assay. Briefly, cells were seeded in 96-well plates at 3 × 103 cells/well. Before treatment with 5-FU, cells were transfected with indicated siRNAs, miRNA mimic and vectors. After 48 h, cells were treated with different doses of 5-FU for 48 h. Then 10 μl MTT solution was added per well at the concentration of 0.5 μg/μl for 4 h. The resulting Formazan was dissolved in DMSO. After agitation of the plate the absorbance was measured at 570 nm by a Varioscan system (Thermo Fisher).
Varioscan system
Manufactured by Thermo Fisher Scientific
The Varioscan system is a spectrophotometric instrument designed for absorbance-based measurements in microplates. It is capable of reading a wide range of wavelengths and can be used for various applications in life science research and drug discovery.
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4 protocols using varioscan system
1
Measuring Cell Viability via MTT Assay
2
Cell Viability Assay in 96-Well Plates
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1500 cells were seeded per well into 96-well plates in triplicates. Cell viability was determined using the Cell Counting Kit-8 (CCK-8, Dojindo) or CellTiter-Glo kits (Promega) according to manufacturer's instructions. Absorbance at 450 nm was determined using a Varioscan system (Thermo Fisher).
3
MTT Assay for Cell Viability
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Cells were seeded at a density of 3000 cells per well in 96-well plates and treated with salicylate at different concentrations for 48 h. Then, 10 μl of MTT solution was added to each well and incubated for 4 h. This assay is based on reducing MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by viable cells to form a purple formazan product. Cells were treated with DMSO to release the formazan and the absorbance was measured at 570 nm using a Varioscan system (Thermo Fisher).
4
Curcumin Cytotoxicity Evaluation by MTT
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Cell viability was analyzed by MTT assays. CRC cells were seeded at a density of 3000 cells per well in 96-well plates. After 24 hours, cells were treated with curcumin at the indicated concentrations for 48 hours, followed by addition of 10 μl MTT solution (5 mg/ml) per well and incubation for 4 hours. Cells were lysed with 100 μl DMSO per well for 15 min to release the resulting formazan according to Twentyman et al. [52 (link)], and analyzed by measuring the absorbance at 570 nm by a Varioscan system (Thermo Fisher).
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