Usp28

Manufactured by Fortis Life Sciences

USP28 is a laboratory equipment product manufactured by Fortis Life Sciences. It is a specialized device used for the detection and quantification of the USP28 protein, which is involved in various cellular processes. The core function of USP28 is to facilitate the analysis and measurement of USP28 levels in biological samples, providing researchers and scientists with valuable data for their studies.

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Lab products found in correlation

Survivin, by R&D Systems (1 mentions) Cyclin a, by Santa Cruz Biotechnology (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Usp10, by Fortis Life Sciences (1 mentions) Cyclin e, by Merck Group (1 mentions) Parp 1 2, by Santa Cruz Biotechnology (1 mentions) Usp9x, by Fortis Life Sciences (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Flag m2, by Merck Group (1 mentions) Usp24, by Proteintech (1 mentions) P15ink4b, by Abcam (1 mentions) P14arf, by Cell Signaling Technology (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions) P16ink4a, by Cell Signaling Technology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

2 protocols using usp28

Comprehensive Antibody Immunoblotting Assay

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The following antibodies were used: beta‐actin (1:10,000, mouse), BIRC8 (mouse, Abnova # H00112401‐B01), cIAP1/2, cleaved caspase‐3‐5A1E (1:300, rabbit, Cell Signaling #9664), CUL1 (1:500, mouse, Invitrogen #32‐2400), cyclin A (1:1,000, mouse, Santa Cruz #sc‐751), cyclin B1 (1:1,000, mouse, Cell Signaling #4138), cyclin E (1:1,000, mouse, kind gift of M. Pagano), FLAG (1:1,000, rabbit, Sigma #F7425), FLAG‐M2 (1:1,000, mouse, Sigma #F3165), HA‐16B12 (1:2,000, mouse, Covance #MMS‐101P), human MCL1 (1:500, rabbit, BD Pharmingen #554103), murine MCL1 (1:1,000, rabbit, A&D Serotec AHP1249), ML‐IAP (mouse, Santa Cruz #sc‐71592), NIAP (rabbit, Abcam #ab25968), PARP1/2 (1:1,000, rabbit, Santa Cruz #sc‐7150), pHH3 (S10) (1:300, rabbit, Cell Signaling #9701), PLK1 (1:500, rabbit, Invitrogen #33‐1700), survivin (1:3,000, rabbit, R&D Systems #AF886), USP7 (rabbit, Bethyl Lab. #A300‐033A), USP9X (1:4,000, rabbit, Bethyl Laboratories #A301‐351A), USP10 (rabbit, Bethyl Lab. #A300‐900A), USP24 (Proteintech Europe, #13126‐1‐AP), USP28 (rabbit, Bethyl Lab. #A300‐898A), V5 (1:1,000, rabbit, Sigma #V8137), XIAP (1:1,000, mouse, BD Biosciences #610716), XIAP (1:1,000, rabbit, Cell Signaling #2042), and XIAP (1:1,000, R&D Systems #AF8221).

Engel K., Rudelius M., Slawska J., Jacobs L., Ahangarian Abhari B., Altmann B., Kurutz J., Rathakrishnan A., Fernández‐Sáiz V., Brunner A., Targosz B., Loewecke F., Gloeckner C.J., Ueffing M., Fulda S., Pfreundschuh M., Trümper L., Klapper W., Keller U., Jost P.J., Rosenwald A., Peschel C, & Bassermann F. (2016). USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B‐cell lymphoma. EMBO Molecular Medicine, 8(8), 851-862.

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Protein Expression Analysis Protocol

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Cell extracts were prepared by lysis in Laemmli buffer in the presence of protease inhibitor cocktail (Roche, Indianapolis, IN). The ZNF304 antibody was generated (by 21^st Century Biochemicals, Marlboro, MA) against a peptide corresponding to amino acids GFWCEAEHEAPSEQSV. The following commercial antibodies were used: p14^ARF (Cell Signaling Technology, Danvers, MA), p15^INK4B (Abcam, Cambridge, MA), p16^INK4A (Cell Signaling Technology), USP28 (Bethyl Laboratories, Montgomery, TX), PRKD1 (Cell Signaling Technology), phospho-ERK1/2 and total ERK1/2 (both from Cell Signaling Technology). The α-tubulin (TUBA) antibody was generated in-house.

Serra R.W., Fang M., Park S.M., Hutchinson L, & Green M.R. (2014). A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. eLife, 3, e02313.

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