MultiDsk2 pull-down assays were performed as previously described with slight modifications4 . Ground cell power was resuspended in D-buffer (150 mM Tris-Acetate pH 7.4, 100 mM potassium acetate, 1 mM EDTA, 0.1% Triton X-100, 10% glycerol, 20 μM MG132 and 50 μM PR-619 Protease Inhibitor (Merck Life Sciences)) and incubated 30’ on ice. After centrifugation, 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher) and subjected to western analysis as described above.
Pr 619 protease inhibitor
Manufactured by Merck Group
PR-619 is a protease inhibitor developed by Merck Group. It functions by inhibiting the activity of proteases, which are enzymes responsible for breaking down proteins. The core purpose of PR-619 is to serve as a research tool for studying protein interactions and regulating cellular processes.
Automatically generated - may contain errors
2 protocols using pr 619 protease inhibitor
1
Multidsk2 Pull-Down Assay Protocol
2
Multidsk2 Pull-Down Assay Protocol
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