Suberoylanilide hydroxamic acid (SAHA) or Vorinostat (Cayman, Germany), Lysotracker Red DND-99 (Invitrogen, Germany), B27 supplement 1:50 (Invitrogen, UK), Anti-E-cadherin (Cell signaling, Danvers, MA, USA), Anti-Snail (Cell signaling, Danvers, MA, USA), Anti-β-actin (Santa Cruz Biotech, CA, USA) primary antibodies and Tetrachloroauric acid (HAuCl4‚3H2O)(Acros organics, USA) were purchased. Sodium borohydride (NaBH4), L-ascorbic acid, Cetyltrimethylammonium bromide (CTAB), Sodium thiosulfate, Silver nitrate (AgNO3), RPMI-Medium (Biosera, France), Fetal bovine serum (FBS)(Gibco, USA), Penicillin/streptomycin (Biowest, France). TWEEN20, Dimethyl sulfoxide (DMSO), and formaldehyde were all purchased from Merck (Merck, Germany). PKF118-310, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), Fluorescein isothiocyanate (FITC), and Human Epidermal Growth Factor were purchased from Sigma Aldrich. Human plasma was obtained from the Blood Transfusion Organization (IRAN) and then aliquoted and stored at −80°C. SAHA was dissolved in deionized water (pH 12, 5 mg/mL) and PKF was dissolved in deionized water (5mg/mL) as stock solutions and were used for further experiments.
Tetrachloroauric acid
Manufactured by Thermo Fisher Scientific
Sourced in United States
Tetrachloroauric acid is a chemical compound used in various laboratory applications. It is a yellow, crystalline solid that serves as a source of gold ions. The compound is commonly used in the preparation of gold nanoparticles and other gold-based materials for research and analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Penicillin streptomycin, by Biowest (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Human epidermal growth factor (hegf), by Merck Group (1 mentions)
Rpmi medium, by Biosera (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Suberoylanilide hydroxamic acid, by Cayman Chemical (1 mentions)
Pkf118 310, by Merck Group (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Vorinostat, by Cayman Chemical (1 mentions)
Anti snail, by Cell Signaling Technology (1 mentions)
Silver nitrate agno3, by Merck Group (1 mentions)
Tecnai g2 f20 s twin, by Thermo Fisher Scientific (1 mentions)
3 protocols using tetrachloroauric acid
1
Novel SAHA-Based Epigenetic Modulation
2
Synthesis and Characterization of Au-Ag Nanoparticles
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All the reagents were reagent grade and used as purchase without further purification. Tetrachloroauric acid (HAuCl4, 0.2 M aqueous solution) and zinc powder were purchased from Acros Organics (New Jersey, USA). Silver nitrate (AgNO3, 0.1 M aqueous solution) was purchased from Merck & Co. (New Jersey, USA). Ultra-pure water was purchased from Hao Feng Biotech Co. (Taipei, Taiwan). All cell culture reagents were obtained from Invitrogen Inc. (Carlsbad, CA, USA).
The transformations in the surface plasmonic resonance of Au-NP and Ag-NP were measured on an Agilent Technologies Cary60 ultraviolet–visible (UV–vis) spectrophotometer operating at a resolution of 2 nm. Infrared (IR) spectra were recorded on an Agilent Technologies Cary630 FT-IR spectrometer. Transmission electron microscopy (TEM) was performed on an FEI Tecnai G2 F-20 S-TWIN operating at an accelerating voltage of 200 kV. Particle size distribution and zeta potential were measured on an Otsuka ELSZ-2000ZS dynamic light scattering (DLS) detector. The content of metal ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) on an Agilent 5100 instrument. All the analyses were carried out with triplicate measurements for a single sample.
The transformations in the surface plasmonic resonance of Au-NP and Ag-NP were measured on an Agilent Technologies Cary60 ultraviolet–visible (UV–vis) spectrophotometer operating at a resolution of 2 nm. Infrared (IR) spectra were recorded on an Agilent Technologies Cary630 FT-IR spectrometer. Transmission electron microscopy (TEM) was performed on an FEI Tecnai G2 F-20 S-TWIN operating at an accelerating voltage of 200 kV. Particle size distribution and zeta potential were measured on an Otsuka ELSZ-2000ZS dynamic light scattering (DLS) detector. The content of metal ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) on an Agilent 5100 instrument. All the analyses were carried out with triplicate measurements for a single sample.
3
Synthesis of Gold Nanoparticles from MT Seeds
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All reagents and chemicals used in this study were used directly, with no further purification. Tetrachloroauric acid (HAuCl4·3H2O, ≥ 99.9%) were purchased from Acros Organics (Belgium). Lead(ii ) nitrate (Pb(NO3)2, ≥ 99%) and other metal were obtained from Beijing Chemical Company (Beijing, China). MT seeds were collected in Ha Giang province of Vietnam and sun-dried until the moisture content was about 10%.
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