Tabletop tm3030

Scanning electron microscopy (SEM) was used to observe the dorsal and ventral surfaces of the butterfly wing for presence of microbes. The unprocessed pair of hind and fore wings was clipped and glued onto copper pin mounts with a double-sided carbon adhesive tape, and SEM imaging was done using a scanning electron microscope (Hitachi Tabletop TM3030; from the Department of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, India). Any microbes observed were imaged.

Egel-20 and Egel-20-NCs were printed into a mesh pattern using a 3D bio-printer (Bioscaffolder 3.2, GeSiM, Radeberg, Germany) equipped with a heated cartridge and a 0.25 ​mm nozzle. Both materials were placed into the cartridge and heated at 90 ​°C for 30 ​min before printing. Then, the temperature was allowed to equilibrate at 41 ​°C, and meshes were printed using a pressure of 300 ​kPa. The printing speed was 5 ​mm/s, and the layer height was 0.25 ​mm. The resultant meshes were observed in a digital microscope (VHX, Keyence, Ltd, Milton Keynes, UK) and a scanning electron microscope (Tabletop TM 3030, Hitachi, Tokyo, Japan). Furthermore, DSC experiments and redispersion of CUR-NCs from the meshes were performed to assess the effect of the 3D printing on the system.

Needles were observed with a Tabletop TM3030 scanning electron microscope (Hitachi, Tokyo, Japan) to evaluate the coating distribution and determine whether a homogenous coating film was formed. Needles were attached to a double-side carbon conductive paper and placed in an aluminium sample holder. Equipment was set in a low vacuum mode at a voltage of 15 kV.

Scanning electron microscopy (SEM) was used to observe the dorsal and ventral surfaces of the butterfly wing for presence of microbes. The unprocessed pair of hind and fore wings was clipped and glued onto copper pin mounts with a double-sided carbon adhesive tape, and SEM imaging was done using a scanning electron microscope (Hitachi Tabletop TM3030; from the Department of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, India). Any microbes observed were imaged.

Polymeric films containing CTAB-GnRs or PEG-GnRs were fabricated using a mixture (%w/w) of 25% Gantrez^® S-97, 10% PEG 200 Da, and q.s. of the concentrated GnRs suspension. For CTAB-GnRs, 50 mL of CTAB-GnRs suspension was centrifuged at 8500 rpm for 45 min and resuspended in the same volume of purified water, centrifuged again, and then concentrated by completing the volume until it reached 5 mL. For PEG-GnRs, the suspension obtained in Section 2 was used directly after dialysis, making up the final volume to 5 mL, as well for comparison purposes. Films without GnRs were also fabricated as controls, using only distilled water instead of GnR suspension. Obtained gels were centrifuged at 5000 rpm for 15 m to remove air bubbles and then cast on a 9 cm² siliconized release liner. Films were left to dry at room temperature for 2 days, cut in approximately 1 cm² pieces and then crosslinked in an oven at 80°C overnight. Films were visualized using digital and a Tabletop TM3030 transmission scanning electron microscope (Hitachi, Tokyo, Japan) and then measured in terms of thickness, area and weight.

The morphology of freeze-dried PLGA-DOXO NPs was analyzed by Scanning Electron Microscopy (SEM, Hitachi TM3030 Tabletop, Germany) at accelerating voltage of 10 kV. SEM images were obtained at a magnification of X 6K and analyzed using an image software (ImageJ) for size measurement. Moreover, the particle size analysis and size distribution analysis were performed by using a dynamic laser light scattering technology (Malvern Zetasizer, Nano ZS) at room temperature in ultrapure water. PLGA-DOXO NPs were suspended in DI water (0.5 mg/mL) with a material refractive index of 1.49 and absorbance of 0.001 to obtain NP hydrodynamic diameter (nm). Results are an average of triplicate samples.