Nf κb2 p100 52

Manufactured by Cell Signaling Technology

Sourced in United States

NF-κB2 (p100/52) is a protein involved in the NF-κB signaling pathway. It functions as a transcription factor that regulates the expression of genes involved in immune and inflammatory responses.

Automatically generated - may contain errors

Lab products found in correlation

Primescript rt reagent kit, by Takara Bio (1 mentions) Sybr green reagent, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Ab128915, by Abcam (1 mentions) Safranal, by Merck Group (1 mentions) Ab108595, by Abcam (1 mentions) Ab32147, by Abcam (1 mentions) Anti β actin antibody 66009 1 ig, by Proteintech (1 mentions) Ab32072, by Abcam (1 mentions) Rela p65, by Cell Signaling Technology (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Phospho rela ser536, by Cell Signaling Technology (1 mentions) Phospho stat1 tyr701, by Cell Signaling Technology (1 mentions) Mhc 1, by Thermo Fisher Scientific (1 mentions) Nf κb1 p105 50, by Cell Signaling Technology (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Pd l1, by Abcam (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

NF-κB2 (13 citations) P100/52 (2 citations) Antibody against NF-κB2 p100/52 (2 citations)

2 protocols using nf κb2 p100 52

Safranal Immunoblotting Technique

Check if the same lab product or an alternative is used in the 5 most similar protocols

Safranal (≥90.0% purity) was purchased from Sigma Aldrich (St. Louis, MO, United States), dissolved in dimethyl sulfoxide (DMSO, Life Technologies, Carlsbad, CA, United States), and stored at -80°C. Propidium iodide (PI, P4170; Sigma-Aldrich), PrimeScript RT Reagent Kit (RR037A; TAKARA Biotechnology, Shiga, Japan), SYBR Green reagent (S-7563; Life Technologies), and SYBR Green Realtime PCR Master Mix (QPK-201; TOBOYO, Life Science, Osaka, Japan) were used. The antibodies for immunoblotting included those against CDK4 (#3830-1), CDK6 (#3524-1), and p21 (#3733-1) (Epitomics, Cambridge, United Kingdom). We acquired antibodies against p27 (sc528), IKKα/β (sc7607), α-tubulin (sc5286), and Skp2 (sc7164) from Santa Cruz Biotechnology (Dallas, CA, United States), while anti-β-actin antibody (#66009-1-Ig) was purchased from Proteintech (Wuhan, Hu Bei Province, China). The antibodies against p-Rb (Ser807/811, #9308), E2F1 (#3742), phospho-AKT (Ser473, #4060), AKT (#9272), phospho-IKKα/β (Ser176/180, #2697), phospho-IκBα (Ser32, #2859), IκBα (#4812), NF-κB p65 (#8242), and NF-κB2 (p100/52, #3017) were purchased from Cell Signaling Technology (Danvers, MA, United States), while those against lamin A/C (ab108595), CDK2 (ab32147), c-MYC (ab32072), and GAPDH (ab128915) were supplied by Abcam (Cambridge, United Kingdom).

Jiang X., Li Y., Feng J.L., Nik Nabil W.N., Wu R., Lu Y., Liu H., Xi Z.C, & Xu H.X. (2020). Safrana l Prevents Prostate Cancer Recurrence by Blocking the Re-activation of Quiescent Cancer Cells via Downregulation of S-Phase Kinase-Associated Protein 2. Frontiers in Cell and Developmental Biology, 8, 598620.

+ Open protocol

+ Expand

Western Blot Analysis of Immune Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer (Thermo Fisher, 89900) supplemented with phosphatase and protease inhibitors and subjected to SDS-PAGE. Antibodies used were ERa (Santa Cruz Biotechnology; cat. #sc-543, RRID:AB_631471), RelA/p65 (Cell Signaling Technology; cat. #6956, RRID:AB_10828935), phospho-RelA Ser536 (Cell Signaling Technology; cat. #3033, RRID:AB_331284), GAPDH (Santa Cruz Biotechnology; cat. #sc-25778, RRID:AB_10167668), RelB (Cell Signaling Technology; cat. #10544, RRID:AB_2797727), NF-κB1 p105/50 (Cell Signaling Technology; cat. #12540, RRID:AB_2687614), NF-κB2 p100/52 (Cell Signaling Technology cat. #3017, RRID:AB_10697356), IRF1 (Cell Signaling Technology; cat. #8478, RRID:AB_10949108), STAT1 (Cell Signaling Technology; cat. #9176, RRID:AB_2240087), phospho-STAT1 Tyr701 (Cell Signaling Technology; cat. #9167, RRID:AB_561284), MHC-I (Thermo Fisher Scientific; cat. #MA5-11723, RRID:AB_10985125), PD-L1 (Abcam; cat. #ab228415, RRID:AB_2884993).

Hermida-Prado F., Xie Y., Sherman S., Nagy Z., Russo D., Akhshi T., Chu Z., Feit A., Campisi M., Chen M., Nardone A., Guarducci C., Lim K., Font-Tello A., Lee I., García-Pedrero J., Cañadas I., Agudo J., Huang Y., Sella T., Jin Q., Tayob N., Mittendorf E.A., Tolaney S.M., Qiu X., Long H., Symmans W.F., Lin J.R., Santagata S., Bedrosian I., Yardley D.A., Mayer I.A., Richardson E.T., Oliveira G., Wu C.J., Schuster E.F., Dowsett M., Welm A.L., Barbie D., Metzger O, & Jeselsohn R. (2023). Endocrine Therapy Synergizes with SMAC Mimetics to Potentiate Antigen Presentation and Tumor Regression in Hormone Receptor–Positive Breast Cancer. Cancer Research, 83(19), 3284-3304.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!