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Sodium azide nan3

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Sodium azide (NaN3) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and other polar solvents. Sodium azide is a powerful reducing agent and is often used in various applications, such as in the synthesis of other compounds and as a preservative in some laboratory solutions.

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Lab products found in correlation

3 trimethylsilyl propionic acid 2 2 3 3 d4 sodium salt tsp, by Thermo Fisher Scientific (6 mentions) Hydrochloric acid (hcl), by Avantor (5 mentions) Sodium phosphate, by Avantor (5 mentions) Deuterium oxide d2o, by Cambridge Isotopes (4 mentions) Milli q advantage water purification system, by Merck Group (4 mentions) Deuterium oxide 2h2o, by Cambridge Isotopes (2 mentions) Thimerosal, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) Trypsin ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions) Hoechst 33342 62249, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions) Rat tail collagen type 1, by Corning (1 mentions) Tween 20, by Merck Group (1 mentions) Sylgard 184 silicone elastomer kit, by Dow (1 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Ab76315, by Abcam (1 mentions) Ab119352, by Abcam (1 mentions)

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Sodium azide (34 citations)

7 protocols using sodium azide nan3

1

NMR Metabolomics Sample Preparation

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Lactulose (Laevolac) was obtained from Fresenius Kabi Austria, D-Mannitol (Fluka), sucrose and thimerosal were obtained from Sigma Aldrich Austria. Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide (2H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Stryeck S., Horvath A., Leber B., Stadlbauer V, & Madl T. (2018). NMR spectroscopy enables simultaneous quantification of carbohydrates for diagnosis of intestinal and gastric permeability. Scientific Reports, 8, 14650.
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2

Preparation of Phosphate NMR Buffer

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Dibasic sodium phosphate (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). From Alfa Aesar (Karlsruhe, Germany), 3(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) was obtained. Deuterium oxide (D2O) was obtained from Cambridge Isotopes laboratories (Tewksbury, MA, USA). Deionized water was purified using the in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). All chemicals were used with no further purification. The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na2HPO4, 0.4 g of TSP, and 0.2 g NaN3 in 400 mL of D2O and adjusted to pH 7.4 with 1M NaOH and HCl. Upon addition of D2O to a final volume of 500 mL, the pH was re-adjusted to pH 7.4 with 1M NaOH and HCl.
Kreuzer K., Reiter A., Birkl-Töglhofer A.M., Dalkner N., Mörkl S., Mairinger M., Fleischmann E., Fellendorf F., Platzer M., Lenger M., Färber T., Seidl M., Birner A., Queissner R., Mendel L.M., Maget A., Kohlhammer-Dohr A., Häussl A., Wagner-Skacel J., Schöggl H., Amberger-Otti D., Painold A., Lahousen-Luxenberger T., Leitner-Afschar B., Haybaeck J., Habisch H., Madl T., Reininghaus E, & Bengesser S. (2022). The PROVIT Study—Effects of Multispecies Probiotic Add-on Treatment on Metabolomics in Major Depressive Disorder—A Randomized, Placebo-Controlled Trial. Metabolites, 12(8), 770.
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3

PDMS-Based Cell Culture Platform

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Poly (dimethylsiloxane) (PDMS) Sylgard 184 silicone elastomer kit was purchased from Dow Corning (Midland, MI) and poly (acrylic acid) (PAA) (MW 30,000, 30% in H2O) from Polysciences Inc. (Warrington, PA). Methanol, bovine serum albumin (BSA), and Triton X-100 were from Fisher Scientific (Hampton, NH). RBL-2H3 (CRL-2256) and H1299 (CRL-5803) cells were purchased from American Type Culture Collection (ATCC) (Manassas, VA). Penicillin/streptomycin (P/S), Minimal Essential Medium (MEM) Alpha, Roswell Park Memorial Institute (RPMI) medium and 0.05% trypsin-Ethylenediaminetetraacetic acid (EDTA) were obtained from Life Technologies (Waltham, MA). Fetal bovine serum (FBS) was from Atlanta Biologicals (Norcross, GA) and sodium azide (NaN3) from VWR (Radnor, PA). Primary antibodies for STAT3 (ab119352) and STAT3 phosphorylated at Y705 (ab76315) were purchased from abcam (Cambridge, MA). Secondary antibody with Alexa Fluor 647 (715-605-150) was from Jackson ImmunoResearch Inc. (West Grove, PA) and with Alexa Fluor 555 (A-31572) from Thermo Fisher (Waltham, MA). Hoechst 33342 (62249) was from Thermo Fisher. Tween-20 was obtained from Sigma-Aldrich (St. Louis, MO). Phosphate buffered saline (PBS) and rat tail collagen type I were purchased from Corning (Corning, NY).
Smiddy N.M., DiSalvo M., Allbritton-King J.D, & Allbritton N.L. (2020). Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies. The Analyst, 145(7), 2649-2660.
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4

NMR Spectroscopy Sample Preparation

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Methanol, sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Deionized water was purified using an in-house Milli-Q® Advantage water purification system from Millipore (Schwalbach, Germany).
Gross A.S., Zimmermann A., Pendl T., Schroeder S., Schoenlechner H., Knittelfelder O., Lamplmayr L., Santiso A., Aufschnaiter A., Waltenstorfer D., Ortonobes Lara S., Stryeck S., Kast C., Ruckenstuhl C., Hofer S.J., Michelitsch B., Woelflingseder M., Müller R., Carmona-Gutierrez D., Madl T., Büttner S., Fröhlich K.U., Shevchenko A, & Eisenberg T. (2019). Acetyl-CoA carboxylase 1–dependent lipogenesis promotes autophagy downstream of AMPK. The Journal of Biological Chemistry, 294(32), 12020-12039.
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5

Preparation of Phosphate NMR Buffer

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Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide (NaOH), hydrochloric acid (HCl, 32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3(Trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotopes Laboratories (Tewksbury, MA, USA). Deionized water was purified using an in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). All chemicals were used without further purification. The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na2HPO4, 0.4 g of TSP, and 0.2 g NaN3, in 400 mL of D2O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D2O to a final volume of 500 mL the pH was re-adjusted to pH 7.4 with 1 M NaOH and HCl.
Reisinger A.C., Posch F., Hackl G., Marsche G., Sourij H., Bourgeois B., Eller K., Madl T, & Eller P. (2021). Branched-Chain Amino Acids Can Predict Mortality in ICU Sepsis Patients. Nutrients, 13(9), 3106.
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6

Preparation of NMR Phosphate Buffer

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Sodium phosphate, dibasic (Na 2 HPO 4 ), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN 3 ) were obtained from VWR International (Darmstadt, Germany). 3(trimethylsilyl) propionic acid-2,2,3,3-d 4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D 2 O) was obtained from Cambridge Isotopes laboratories (Tewksbury, MA). Deionized water was purified using in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na 2 HPO4, 0.4 g of TSP, and 0.2 g NaN 3 , in 400 mL of D 2 O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D 2 O to a final volume of 500 mL, the pH was readjusted to pH 7.4 with 1 M NaOH and HCl.
Streese L., Springer A.M., Deiseroth A., Carrard J., Infanger D., Schmaderer C., Schmidt-Trucksäss A., Madl T, & Hanssen H. (2021). Metabolic profiling links cardiovascular risk and vascular end organ damage. Atherosclerosis, 331.
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7

Quantitative Metabolite Profiling

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L-arginine was obtained from AppliChem (Germany), ω-N G , N G -asymmetric dimethylarginine (ADMA), ω-N G -N' G -symmetric dimethylarginine (SDMA), and ω-N G -monomethylarginine (MMA) were obtained from Santa Cruz Biotechnology, Inc. (Germany). Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, chloroform, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide ( 2 H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.
Zhang F., Kerbl-Knapp J., Colman M.J.R., Macher T., Vujić N., Fasching S., Jany-Luig E., Korbelius M., Kuentzel K.B., Mack M., Akhmetshina A., Paar M., Rinner B., Hörl G., Steyrer E., Stelzl U., Burgering B., Eisenberg T., Pertschy B., Kratky D., & Madl T. (2021). Global analysis of protein arginine methylation.
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