Anti gr1 fitc rb6 8c5

Manufactured by BioLegend

Anti-Gr1 FITC (RB6-8C5) is a fluorescently-labeled antibody that binds to the Gr-1 (Ly-6G/Ly-6C) antigen. The Gr-1 antigen is expressed on the surface of granulocytes, including neutrophils, eosinophils, and myeloid-derived suppressor cells (MDSCs).

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Lab products found in correlation

Anti cd45 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd11c pe, by BioLegend (1 mentions) Anti 1 a 1 e pacific blue, by BioLegend (1 mentions) Anti f4 80 pe cyanine7, by Thermo Fisher Scientific (1 mentions) Anti cd14 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd19 apc, by BioLegend (1 mentions) Anti cd11b percp cy5, by Thermo Fisher Scientific (1 mentions) Moflo astrios eq, by Beckman Coulter (1 mentions) Novocyte, by Agilent Technologies (1 mentions) Lsrfortessa, by BD (1 mentions)

2 protocols using anti gr1 fitc rb6 8c5

Multicolor Flow Cytometry Immunophenotyping

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After blocking cells were incubated with live/dead cell staining (Live/Dead Aqua 405, Invitrogen) for 15 minutes on ice. Cell surface staining occurred on ice for 30 minutes. All samples were acquired on an LSR 4/5 laser Fortessa (BD) and data analyzed using FlowJo v10. Human antibody: anti-CD14 FITC (61D3, Invitrogen), anti-CD45 APC-eFluor780 (HI30, eBioscience), anti-CD19 eFluor450 (HIB19, eBioscience), anti-CD3 BV785 (OKT3, BioLegend), anti-CD8 PE (HIT8a, BioLegend), anti-CD16 PE-Cyanine7 (CB16, Invitrogen). Murine antibody (myeloid panel): anti-Gr1 FITC (RB6-8C5, BioLegend), anti-CD11b PerCP-Cy5.5 (M1/70, eBioscience), anti-CD3e APC (145-2C11, eBioscience), anti-CD19 APC (1D3, BioLegend), anti-CD45 APC-eFluor780 (30-F11, Invitrogen), anti-I-A/I-E Pacific Blue (M5/114.15.2, Biolegend), anti-CD11c PE (N418, BioLegend), anti-F4/80 PE-Cyanine7 (BM8, Invitrogen). Murine antibody (lymphoid panel): anti-CD4 FITC (GK1.5, eBioscience), anti-CD19 APC (1D3, BioLegend), anti-CD45 APC-eFluor780 (30-F11, Invitrogen), anti-NK1.1- Pacific Blue (PK136, BioLegend), anti-CD8a BV785 (53-6.7, BioLegend), anti-NKp46 PE/Dazzle (29A1.4, BioLegend), anti-CD3 PE-Cyanine7 (17A2, BD PharMingen).

Tuong Z.K., Loudon K.W., Berry B., Richoz N., Jones J., Tan X., Nguyen Q., George A., Hori S., Field S., Lynch A.G., Kania K., Coupland P., Babbage A., Grenfell R., Barrett T., Warren A.Y., Gnanapragasam V., Massie C, & Clatworthy M.R. (2021). Resolving the immune landscape of human prostate at a single-cell level in health and cancer. Cell Reports, 37(12), 110132.

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Flow Cytometry Immune Cell Profiling

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The antibodies and reagents used for surface staining were fixed viability dye, anti-CD45 (APC-Cyanine7, 30-F11), anti-CD11b (PE, N418), anti-Gr-1 (FITC, RB6–8C5), and anti-F4/80 (APC-F4/80), all from BioLegend. Samples were run on an LSR Fortessa (BD) or NovoCyte (ACEA). Cell sorting was performed by Beckman Moflo Astrios EQ. Data were analyzed using FlowJo v10 software.

Zhu K., Jin X., Chi Z., Chen S., Wu S., Sloan R.D., Lin X., Neculai D., Wang D., Hu H, & Lu L. (2021). Priming of NLRP3 inflammasome activation by Msn kinase MINK1 in macrophages. Cellular and Molecular Immunology, 18(10), 2372-2382.

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