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Lightsnip kit

Manufactured by TIB Molbiol
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Sourced in Germany

The LightSNiP kit is a compact and automated system designed for single nucleotide polymorphism (SNP) detection and analysis. The kit utilizes a proprietary technology to enable rapid and reliable SNP genotyping with minimal hands-on time.

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Lab products found in correlation

Light cycler 480 software 1, by Roche (1 mentions) Faststart dna master hybprobe, by Roche (1 mentions) Lightcycler 480 2, by Roche (1 mentions) High pure pcr template preparation kit, by Roche (1 mentions)

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LightSNiP (12 citations)

2 protocols using lightsnip kit

1

Genotyping of BDNF and VEGF Polymorphisms

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Genomic DNA was extracted from peripheral blood using standard methods. The SNP rs6265 in BDNF and rs699947 in the promotor region of VEGF were genotyped by a real-time polymerase chain reaction with melting curve analysis using a LichtCycler 480 II instrument (Roche, Penzberg, Germany). For each SNP a LightSNiP kit (TIB Molbiol, Berlin, Germany) was used with pre-mixed primer and probes specific for each SNP. Melting temperatures were called using Lightcycler 480 Software 1.5.1 (Roche, Penzberg, Germany).
Van Den Bossche M.J., Emsell L., Dols A., Vansteelandt K., De Winter F.L., Van den Stock J., Sienaert P., Stek M.L., Bouckaert F, & Vandenbulcke M. (2019). Hippocampal volume change following ECT is mediated by rs699947 in the promotor region of VEGF. Translational Psychiatry, 9, 191.
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2

COMT rs4680 Polymorphism Genotyping

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Genomic DNA was extracted from 200 mL of EDTA anticoagulated whole blood samples using the High Pure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany), following the instructions of the manufacturer. The COMT rs4680 polymorphism was genotyped using the LightSNiP Kit (TIB Molbiol, Berlin, Germany) and FastStart DNA Master HybProbe (Roche Diagnostics, Mannheim, Germany). Amplification by real-time PCR and subsequent analysis of melting curve were performed on the LightCycler 480 II (Roche Diagnostics, Mannheim, Germany), according to the manufacturer’s instructions.
Nešković N., Marczi S., Mandić D., Mraovic B., Škiljić S., Kristek G., Vinković H, & Kvolik S. (2021). ANALGESIC EFFECT OF TRAMADOL IS NOT ALTERED BY POSTOPERATIVE SYSTEMIC INFLAMMATION AFTER MAJOR ABDOMINAL SURGERY. Acta Clinica Croatica, 60(2), 268-275.
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