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Peroxidase conjugated anti fam

Manufactured by Roche
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The Peroxidase-conjugated anti-FAM is a laboratory reagent used in various biochemical and molecular biology applications. It consists of an antibody specific to the fluorescent dye FAM (Carboxyfluorescein), which is conjugated to the enzyme peroxidase. This conjugate can be used to detect and quantify the presence of FAM-labeled biomolecules, such as DNA, RNA, or proteins, through colorimetric or chemiluminescent detection methods.

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Lab products found in correlation

Fitc conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Mounting medium, by Thermo Fisher Scientific (1 mentions) Cy3 conjugated goat anti rabbit, by Jackson ImmunoResearch (1 mentions)

3 protocols using peroxidase conjugated anti fam

1

miR-146 Expression Profiling with FISH and IHC

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FISH combined with immunohistochemical staining were performed, as previously described [36 (link)]. Briefly, the sections were hybridized with miR-146 LNA probe, and the probe was detected with peroxidase-conjugated anti-FAM (Roche) followed by incubation tyramine-signal-amplification (TSA)-Cy3 substrate for 10 min at room temperature. Primary antibody was added and incubated at 4 degrees overnight and detected with FITC-conjugated secondary antibody (Jackson ImmunoResearch). Slides were mounted with mounting medium with DAPI (Invitrogen).
Liu X.S., Chopp M., Pan W.L., Wang X.L., Fan B.Y., Zhang Y., Kassis H., Zhang R.L., Zhang X.M, & Zhang Z.G. (2016). MicroRNA-146a promotes oligodendrogenesis in stroke. Molecular neurobiology, 54(1), 227-237.
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2

Quantifying miR-21 and HER2 in Tissues

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MicroRNA-21 ISH combined with immunohistochemical staining for HER2 was performed essentially as described (35 (link)). After proteinase-K treatment, sections were hybridized with 20 nM miR-21 LNA probe for 1 h, and the probe was detected with peroxidase-conjugated anti-FAM (Roche) followed by incubation tyramine-signal-amplification (TSA)-Cy5 substrate for 5 min at room temperature. Polyclonal rabbit anti-ErbB-2 (ab2428, AbCam, Cambridge, UK) was incubated at room temperature and detected with Cy3-conjugated goat anti rabbit (Jackson ImmunoResearch, West Grove, PA, USA). Slides were mounted with Antifade Gold with DAPI (Invitrogen).
Nielsen B.S., Balslev E., Poulsen T.S., Nielsen D., Møller T., Mortensen C.E., Holmstrøm K, & Høgdall E. (2014). miR-21 Expression in Cancer Cells may Not Predict Resistance to Adjuvant Trastuzumab in Primary Breast Cancer. Frontiers in Oncology, 4, 207.
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3

miRNA Detection in Neuroblasts

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Fluorescence in situ hybridization (FISH) in combination with fluorescent immunostaining were performed according to a published protocol [30 (link)]. Briefly, the cells were fixed in 4% paraformaldehyde, and then hybridized with a miR-34a LNA (locked nucleic acid) probe, and the probe was detected with peroxidase-conjugated anti-FAM (Roche, Basel, Switzerland) followed by incubation tyramine-signal-amplification (TSA)-Cy3 substrate for 10 min at room temperature. Fluorescent immunostaining was then performed with primary antibodies against TUJ1 (a marker of neuroblasts) and FITC-conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA).
Jia L., Chopp M., Wang L., Lu X., Zhang Y., Szalad A, & Zhang Z.G. (2018). MiR-34a Regulates Axonal Growth of Dorsal Root Ganglia Neurons by Targeting FOXP2 and VAT1 in Postnatal and Adult Mouse. Molecular neurobiology, 55(12), 9089-9099.
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