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Copper 2 sulfate pentahydrate

Manufactured by Fujifilm
Sourced in Japan

Copper(II) sulfate pentahydrate is an inorganic chemical compound with the formula CuSO4·5H2O. It is a blue crystalline solid that is widely used in various laboratory applications. The compound consists of copper(II) ions, sulfate ions, and five water molecules. Its core function is to serve as a source of copper ions and as a reagent in various chemical reactions and analyses.

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5 protocols using copper 2 sulfate pentahydrate

1

Copper-Catalyzed Azide-Alkyne Cycloaddition

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3-Bromo-1-propyne (BrP), sodium azide (NaN3), copper(II) sulfate pentahydrate (CuSO4·5H2O), and sodium (+)-ascorbate (NaAsc) were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Poly(ethylene glycol) (PEG) monomethyl ether (Mn ~750 and 2000, EG18 and EG45, respectively) and N,N-dimethylformamide (DMF) (≥99.9%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Solvents (e.g., acetone, toluene, dichloromethane, diethyl ether, and DMF), were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Water was purified with a Millipore Milli-Q system. All the reagents were used without further purification.
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2

Synthesis of Naphthalene-Based Fluorescent Dyes

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N,N-Dimethylethylenediamine
and succinic acid disodium salt
were purchased from Nacalai Tesque (Kyoto, Japan). 1,8-Naphthalic
anhydride, 3-nitro-1,8-naphthalic anhydride, iodomethane, and methoxypolyethylene
glycol amine 750 were purchased from Sigma-Aldrich Japan (Tokyo, Japan).
4-Bromo-1,8-naphthalic anhydride, naphthalene-1,4,5,8-tetracarboxylic
dianhydride, and sodium methoxide were purchased from Tokyo Chemical
Industries (Tokyo, Japan). Copper(II) sulfate pentahydrate and sodium
polyacrylate (DP: 22,000–70,000) were purchased from Wako Pure
Chemical Industries (Osaka, Japan). Other reagents were of commercial
grade and used without further purification.
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3

Separation and Quantification of Lactate Isomers

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The wild-type, chromosomal integrant, and ldh-converted mutant were
cultured overnight in SMY medium. After centrifugation, the supernatant was purified with
(Carrez I and II), potassium hexacyanoferrate(II) trihydrate and zinc sulfate heptahydrate
(Wako) as described previously [35 (link)]. HPLC (Shimadzu
Corporation) was performed using SUMICHIRAL OA-5000 and OA-5000L columns to separate and
measure L-lactate and D-lactate under the following conditions: mobile phase, 2 mM copper
(II) sulfate pentahydrate (Wako); flow rate, 1.0 mL/min; oven temperature, 40°C; UV
detection, 254 nm.
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4

Electrochemical Copper Deposition Protocol

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PET substrates were purchased
from Toray Industries, Inc., Japan. The Ag paste was purchased from
KAKEN TECH Co., Ltd., Japan. Copper(II) sulfate pentahydrate (CuSO4·5H2O, 99.0%), ascorbic acid (C6H8O6, 99.0%), sodium hydroxide (NaOH, 99.0%),
and ammonia (NH3 aq. 28 w%) were all purchased from FUJIFILM
Wako Pure Chemical Corporation, Ltd., Japan. All of the chemicals
were used as received without further purification. Deionized water
was used for all of the experiments.
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5

Antioxidant Properties of Krill Extract

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Isada krill frozen and packed in a 1-kg plastic shrink wrap was purchased from Hamaichi (Wakayama, Japan) and stored in a freezer at -30°C until use. The average mass and length of the krill were 0.07±0.01 g and 18.00±0.65 mm, respectively. Diethyl ether, Folin-Ciocalteu reagent, trisodium citrate dihydrate, copper (II) sulfate pentahydrate, bovine serum albumin (BSA), 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothaiazoline-6-sulfuric acid) (ABTS), 6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (Trolox), and potassium persulfate, and uracil were purchased from Wako Pure Chemical Industries (Osaka, Japan). Myoglobin, tri -tyrosine, di-L-phenylalanine, and L-phenylalanyl-L-alanine dihydrate were purchased from Sigma-Aldrich (Tokyo, Japan). Glucose oxidase was purchased from Nacalai Tesque (Kyoto, Japan). Other reagents were of analytical grade.
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