Mir 124 3p inhibitor

Small interfering RNA (siRNA) targeting APJ (siRNA-APJ, sequence: CTGACATGTTACTTCTTCA), siRNA-APJ negative control (NC), miR-124-3p mimic, NC mimic, miR-124-3p inhibitor, and NC inhibitor were synthesized by RiboBio Co. (Guangzhou, China). CTDSP1 overexpression adenovirus and the CTDSP1 overexpression adenovirus negative control (NC) were synthesized by GeneChem. (Shanghai, China). Neurons were transfected with siRNA-APJ, siRNA-APJ NC, miR-124-3p mimic, NC mimic, miR-124-3p inhibitor, NC inhibitor, CTDSP1 overexpression adenovirus, or CTDSP1 overexpression adenovirus NC using transfection reagent according to the manufacturer’s instructions (RiboBio Co, China). During this period, neurons were incubated in culture medium without the penicillin/streptomycin solution.

The small interfere- (si-) NC, si-SNHG14, miR-124-3p inhibitor, miR-124-3p mimics, miR-NC, and pcDNA-MMP2 were bought from RiboBio. When HK-2 cells grew 80%-90% confluence, the above transcripts were transfected into HK-2 cells with Lipofectamine 3000 (Invitrogen) for 48 h.

miR-124-3p mimic, miRNA negative control (miR-NC), and miR-124-3p inhibitor were purchased from RiboBio (RiboBio, Guangzhou, China). Small interfering RNAs (siRNAs) against RP11-395G23.3 (si-RP11-395G23.3) and negative control siRNA (siControl) were purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). The sequences were: siControl: 5′-UUCUCCGAACGUGUCACGUTT-3′; si-RP11-395G23.3: 5′-GGAGUUCUCCACAUGUAAATT-3′. They were transfected into Nthy-ori 3-1, C643, and HTh-7 cells by Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific, Inc.), according to the manufacturer’s protocols.