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P rb ser870 811

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P-RB (Ser870/811) is a laboratory reagent that detects the phosphorylation of retinoblastoma protein (Rb) at serine residues 870 and 811. Phosphorylation at these sites is involved in the regulation of cell cycle progression. This product is intended for research use only.

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Lab products found in correlation

Cleaved caspase 3 asp175 5a1e, by Cell Signaling Technology (2 mentions) Phospho p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Cyclin b1, by Cell Signaling Technology (2 mentions) Cyclin d1, by Cell Signaling Technology (2 mentions) P cdk2, by Cell Signaling Technology (2 mentions) Cyclin a2, by Cell Signaling Technology (2 mentions) Pi3 kinase p110α c73f8, by Cell Signaling Technology (2 mentions) Cleaved caspase 8 asp391 18c8, by Cell Signaling Technology (2 mentions) Akt pan 40d4, by Cell Signaling Technology (2 mentions) Phospho akt ser473 d9e xp, by Cell Signaling Technology (2 mentions) P44 42 mapk erk1 2 137f5, by Cell Signaling Technology (2 mentions) Cyclin e, by Cell Signaling Technology (2 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions)

2 protocols using p rb ser870 811

1

Western Blot Analysis of Cell Signaling

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For direct IB analysis, the cells were lysed in radioimmunoprecipitation assay (RIPA) buffer with phosphatase inhibitors. The following primary antibodies were used against: cleaved caspase-3 (Asp175) (5A1E) (#9664), cleaved caspase-8 (Asp391) (18C8) (#9496), RB (#9309), p-RB (Ser870/811) (#8516), CDK2 (#2546), p-CDK2 (#2561), Bcl-2 (#15071), Bax (#5023), p21 (#2947), p27 (#3686), cyclin D1 (#55506), cyclin E (#4136), p44/42 MAPK (Erk1/2) (137F5) (#4695), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP (#4370), Akt (pan) (40D4) (#2920), phospho-Akt (Ser473) (D9E) XP (#4060), PI3 kinase p110α (C73F8) (#4249), cyclin A2 (#91500), and cyclin B1 ( #4135), all purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies to CDK4 (#11026-1-AP), CDK6 (#14052-1-AP), SKP2 (#15010-1-AP), p53 (1C12) (#2524), P16-INK4A (#10883-1-AP), EGFR (#18986-1-AP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (#60004-1-Ig) were purchased from Proteintech (Rosemont, IL, USA). The anti-FLAG was obtained from Sigma-Aldrich (St. Louis, MO, USA). All secondary antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA.
Chen T., Liu L., Zou Y., Hu X., Zhang W., Zhou T., Luo X., Fu W, & Xu J. (2021). Nobiletin downregulates the SKP2-p21/p27-CDK2 axis to inhibit tumor progression and shows synergistic effects with palbociclib on renal cell carcinoma. Cancer Biology & Medicine, 18(1), 227-244.
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2

Immunoblot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For direct IB analysis, cells were lysed in Radio-Immunoprecipitation Assay (RIPA) buffer with phosphatase inhibitors. The following primary antibodies were used: Cleaved Caspase-3 (Asp175) (5A1E) (#9664), Cleaved Caspase-8 (Asp391) (18C8) (#9496), RB (#9309), p-RB (Ser870/811) (#8516), CDK2 (#2546), p-CDK2 (#2561), Bcl-2 (#15071), Bax (#5023), p21 (#2947), p27 (#3686), Cyclin D1 (#55506), Cyclin E (#4136), p44/42 MAPK (Erk1/2) (137F5) (#4695), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP (#4370), Akt (pan) (40D4) (#2920), Phospho-Akt (Ser473) (D9E) XP (#4060), PI3 Kinase p110α (C73F8) (#4249), Cyclin A2 (#91500) and Cyclin B1 ( #4135) were purchased from Cell Signaling Technology. CDK4 (#11026-1-AP), CDK6 (#14052-1-AP), SKP2 (#15010-1-AP), p53 (1C12) (#2524), P16-INK4A (#10883-1-AP), EGFR (#18986-1-AP) and GAPDH (#60004-1-Ig) were purchased from Proteintech. FLAG was obtained from Sigma-Aldrich (# F1804). As well as all secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
Chen T., Liu L., Hu X., Wang K., Zhou T., Luo X., Li L., Fu W., & Xu J. (2020). Nobiletin Down-regulates SKP2-p21/p27-CDK2 Axis to Inhibit Tumor Progression and Shows Synergistic Effect with Palbociclib on Renal Cell Carcinoma.
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