RNA extraction, RNA retrotranscription, real-time PCR and western blot analysis were performed as previously described (Harlan, Pehar, Killoy, & Vargas, 2019 (link)). Membranes were incubated overnight with one of the following antibodies: FABP7 (Thermo Fisher, Cat#: PA5-24949, TL2690549A), Actin (Sigma, A5441, lot: 061M4808) or GADPH (Sigma, SAB2100894, lot: QC9353). Image acquisition was performed in a chemiluminescent western blot scanner (Li-Cor) or exposed on Kodak BioMax Light film. Quantifications were performed using the Image Studio Software (Li-Cor) or the ImageJ Software (NIH). ChIP was performed with the SimpleChIP enzymatic chromatin IP kit (Cell Signaling) using an NF-κB p65 antibody (Cell Signaling, 8242, lot: 13) and analyzed by real-time PCR.
Chemiluminescent western blot scanner
Manufactured by LI COR
The Chemiluminescent Western Blot Scanner is a lab equipment product that captures and analyzes chemiluminescent signals generated from Western blot experiments. It is designed to detect and quantify protein levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Biomax light film, by Kodak (3 mentions)
Image studio software, by LI COR (3 mentions)
Anti actin, by Merck Group (2 mentions)
Anti nr1d1, by Novus Biologicals (2 mentions)
Nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Simplechip enzymatic chromatin ip kit, by Cell Signaling Technology (1 mentions)
Gadph, by Merck Group (1 mentions)
Fabp7, by Thermo Fisher Scientific (1 mentions)
3 protocols using chemiluminescent western blot scanner
1
Protein Extraction and Analysis Workflow
2
Protein Expression Analysis Protocol
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RNA extraction, RNA retrotranscription, real‐time PCR, and western blot analysis were performed as previously described.36 Membranes were incubated overnight with anti‐NR1D1 (Novus, H00009572‐M02) and anti‐Actin (Sigma, A5441, lot: 061M4808). Image acquisition was performed in a chemiluminescent western blot scanner (Li‐Cor) or exposed on Kodak BioMax Light film. The western blot image presented in Figure 4A was quantified using ImageJ Software (NIH), all other western blot quantifications were performed using the Image Studio Software (Li‐Cor).
3
Comprehensive protein analysis protocol
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