The largest database of trusted experimental protocols

Western blot detection reagent ecl solution

Manufactured by Advansta
Sourced in United States

The Western blot detection reagent (ECL solution) is a chemiluminescent substrate used for the detection of proteins in Western blot analysis. It is a two-component system that, when combined, produces a luminescent signal proportional to the amount of target protein present on the membrane. The ECL solution is designed to provide a sensitive and reliable method for visualizing protein bands on a Western blot.

Automatically generated - may contain errors

2 protocols using western blot detection reagent ecl solution

1

Novel Compound Synthesis and Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
1-Methyl-4-phenylpyridium (MPP⁺) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dichlorofluorescein diacetate (DCF-DA), DAPI (4′,6-diamidino-2-phenylindole), Alexa Floure 488, and Alexa Flour 568 were obtained from Invitrogen (Portland, OR, USA). Western blot detection reagent (ECL solution) was supplied by Advansta Inc. (San Jose, CA, USA). MHY2699 was synthesized by Prof. Hyung Ryong Moon (College of Pharmacy, Pusan National University, Busan, Korea), as described elsewhere [18 (link)].
+ Open protocol
+ Expand
2

Mitochondrial Dysfunction and Oxidative Stress Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), MPP+ (1-methyl-4-phenylpyridium), MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide), rotenone, antimycin A, CCCP (carbonyl cyanide 3-chlorophenylhydrazone), oligomycin, and corn oil were obtained from Sigma-Aldrich (St. Louis, MO, USA). Evernic acid (EA) was purchased from Santa Cruz Technology (Santa Cruz, CA, USA). DCF-DA (2′-7′-di-chlorofluorescein diacetate), MitoTrackerTM Red CMXRos, Alexa Flour 488, Alexa Flour 568, and DAPI (4’,6-diamidino-2-phenylindole) were from Invitrogen (Carlsbad, CA, USA), and Western blot detection reagent (ECL solution) was purchased from Advansta (San Jose, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!