Alexa fluor 647 conjugated goat anti human igg h l

Manufactured by Thermo Fisher Scientific

Alexa Fluor-647-conjugated goat anti-human IgG (H+L) is a secondary antibody used for detection and quantification of human immunoglobulin G (IgG) in various immunoassay techniques. The antibody is conjugated to the Alexa Fluor-647 fluorescent dye, which enables visualization and analysis using compatible instrumentation.

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Lab products found in correlation

Lsrii cytometer, by BD (1 mentions)

Close spelling variants of this product

Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs Alexa Fluor ™ 647-conjugated goat anti-human IgG (H + L) antibody Alexa Fluor 647‐conjugated anti‐human IgG (H + L; Alexa-Fluor-647-conjugated goat anti-human IgG Alexa Fluor 647 goat anti-human IgG Alexa Fluor 647 anti-goat IgG Alexa Fluor 647 anti-human IgG Alexa Fluor IgG H + L Anti-IgG Alexa Fluor 647 Anti-human (Alexa Fluor 647;

2 protocols using alexa fluor 647 conjugated goat anti human igg h l

Characterization of SARS-CoV-2 Antibody Responses

Cited 4 times

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Plasma from SARS-CoV-2-infected and uninfected donors were collected, heat-inactivated for 1 hour at 56°C and stored at −80°C until ready to use in subsequent experiments. Plasma from convalescent adult donors S002 (male, 65 years old, 25 days post-symptom onset) and S006 (male, 30 years old, 41 days post-symptom onset) were previously characterized.¹⁶ (link) Plasma from uninfected donors were used as negative controls and used to calculate the seropositivity threshold in the ELISA assay. The monoclonal antibody CR3022 was used as a positive control in ELISA assays as described.⁶⁵ (link) The anti-SARS-CoV-2 Spike CV3-1 and CV3-25 mAbs were isolated from the blood of convalescent donor S006 [CV3] using a fluorescent recombinant stabilized Spike ectodomain to probe Spike-specific B cells as previously reported.⁶⁶ (link) Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments. Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect antibody binding in flow cytometry experiments. The anti-SARS-CoV-2 RBD rabbit antiserum was used for immunoprecipitation experiments and was previously reported.¹⁷ (link)

Chen Y., Prévost J., Ullah I., Romero H., Lisi V., Tolbert W.D., Grover J.R., Ding S., Gong S.Y., Beaudoin-Bussières G., Gasser R., Benlarbi M., Vézina D., Anand S.P., Chatterjee D., Goyette G., Grunst M.W., Yang Z., Bo Y., Zhou F., Béland K., Bai X., Zeher A.R., Huang R.K., Nguyen D.N., Sherburn R., Wu D., Piszczek G., Paré B., Matthies D., Xia D., Richard J., Kumar P., Mothes W., Côté M., Uchil P.D., Lavallée V.P., Smith M.A., Pazgier M., Haddad E, & Finzi A. (2022). Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD. iScience, 26(1), 105783.

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Flow Cytometry-based SARS-CoV-2 Spike Assay

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The flow cytometry-based assay evaluated in this study was developed and conducted by CRCHUM as described previously (43 (link)). Briefly, HEK293T cells transfected with an expression plasmid for the SARS-CoV-2 spike were stained with serological specimens diluted at 1:250. Transfected cells were stained with anti-RBD-Cr3002 monoclonal antibody diluted at 5 μg/mL as a positive control. Alexa Fluor-647-conjugated goat anti-human IgG (H+L) (Invitrogen, Rockford, IL) was used as a secondary antibody to detect IgG bound to SARS-CoV-2 spike. An LSRII cytometer (BD Biosciences, Mississauga, ON, Canada) was used to acquire the specimens. FlowJo v10.5.3 (Tree Star, Ashland, OR) was used for data analysis. A specimen was considered positive for nAbs if the proportion of cells bound by anti-spike antibodies was greater than the seropositivity threshold, which was calculated as the mean median fluorescence intensity (MFI) of all COVID-19-negative plasma plus 3 standard deviations of the mean MFI of all COVID-19-negative plasma plus interassay coefficient of variability.

Valcourt E.J., Manguiat K., Robinson A., Lin Y.C., Abe K.T., Mubareka S., Shigayeva A., Zhong Z., Girardin R.C., DuPuis A., Payne A., McDonough K., Wang Z., Gasser R., Laumaea A., Benlarbi M., Richard J., Prévost J., Anand S.P., Dimitrova K., Phillipson C., Evans D.H., McGeer A., Gingras A.C., Liang C., Petric M., Sekirov I., Morshed M., Finzi A., Drebot M, & Wood H. (2021). Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays. Microbiology Spectrum, 9(3), e00886-21.

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