Cytokine and chemokine analysis were completed to validate RNAseq
findings. A total of n = 36 LTRs (n = 19 CLAD and n = 17 non-CLAD) BAL
supernatant samples were used to detect cytokines and chemokines using Meso
Scale Discovery (MSD) U-PLEX multiplex assay platforms (Meso Scale Discovery,
Rockville, MD).16 (link),17 (link) These BAL samples were obtained from the
same bronchoscopy as the brush samples. We analyzed the cytokines: (Type 1)
IFNγ, TNFα, IL-2, IL-12/IL-23p40, IL-15, IL-18, IL-27,
IL-1β and (Type 2): IL-4, IL-5. IL-6. IL-10, IL-13, GM-CSF and IL-17A,
IL-21, IL-22, and G-CSF, IL1α, IFN-α2a, IFNβ. We analyzed
the following chemokines: CXCR3 family (MIG, IP-10, I-TAC) and CCR5 family
(MIP-1α, MIP–1β, RANTES) and IL-8 (CXCL8). Limit of
detection measured by MSD assay for the cytokines was between 0.08 and 9.6 pg/ml
and for the chemokines was between 0.15 and 7.7 pg/ml.
findings. A total of n = 36 LTRs (n = 19 CLAD and n = 17 non-CLAD) BAL
supernatant samples were used to detect cytokines and chemokines using Meso
Scale Discovery (MSD) U-PLEX multiplex assay platforms (Meso Scale Discovery,
Rockville, MD).16 (link),17 (link) These BAL samples were obtained from the
same bronchoscopy as the brush samples. We analyzed the cytokines: (Type 1)
IFNγ, TNFα, IL-2, IL-12/IL-23p40, IL-15, IL-18, IL-27,
IL-1β and (Type 2): IL-4, IL-5. IL-6. IL-10, IL-13, GM-CSF and IL-17A,
IL-21, IL-22, and G-CSF, IL1α, IFN-α2a, IFNβ. We analyzed
the following chemokines: CXCR3 family (MIG, IP-10, I-TAC) and CCR5 family
(MIP-1α, MIP–1β, RANTES) and IL-8 (CXCL8). Limit of
detection measured by MSD assay for the cytokines was between 0.08 and 9.6 pg/ml
and for the chemokines was between 0.15 and 7.7 pg/ml.