Dna pkcs

Manufactured by Novus Biologicals

DNA-PKcs is a serine/threonine protein kinase that plays a critical role in the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair. It is a key component of the DNA damage response and is involved in the regulation of various cellular processes, including DNA repair, cell cycle progression, and apoptosis.

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Lab products found in correlation

Vinculin, by Cell Signaling Technology (1 mentions) Ponceau s, by Merck Group (1 mentions) Kwikquant image analyzer, by Kindle Biosciences (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Bio-Rad (1 mentions) Xrcc5, by Santa Cruz Biotechnology (1 mentions) Yh2ax, by Cell Signaling Technology (1 mentions)

2 protocols using dna pkcs

Immunoblotting Protocol for DNA Damage Signaling

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Immunoblotting was done as previously described (15 (link)). Polyvinylidene difluoride membranes were stained with Ponceau S (Sigma-Aldrich, P3504) following transfer to confirm equal transfer and loading. Antibodies for DNA-PK-pS2056 (Cat # ab18192, RRID:AB_869495) and actin (Cat # ab49900, RRID:AB_867494) were obtained from Abcam (Cambridge, England). Antibodies for ERK 44/42 pT202/pY204 (Cat # 4370, RRID:AB_2315112), ERK (Cat # 4695, RRID:AB_390779), and vinculin (Cat # 13901, RRID:AB_2728768) were purchased from Cell Signaling Technology (Beverley, MA). DNA-PKcs (Cat # NB600-1203, RRID:AB_2170650) and ATM (Cat # NB100-309, RRID:AB_2243346) antibodies were purchased from Novus Biologicals (Littleton, CO). Antibodies for PKCδ (Cat # 14188-AP-1, RRID:AB_10638614) and ATM-pS1981 (Cat # AF1655, RRID:AB_2062935) were purchased from Proteintech (Rosemont, IL) and R&D Systems (Minneapolis, MN), respectively. Chemiluminescent images were captured digitally using the KwikQuant Image Analyzer (Kindle Biosciences, Greenwich, CT) and area density analysis performed using the KwikQuant analysis software.

Affandi T., Ohm A.M., Gaillard D., Haas A, & Reyland M.E. (2021). Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair. The Journal of Biological Chemistry, 296, 100401.

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Antibody Usage in Western Blotting and ChIP

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We used the following commercial antibodies: JARID2 (NB100-2214; Novus Biologicals, Littleton, CO), CYLD (sc-28211; Santa Cruz Biotechnology, Santa Cruz, CA), XRCC5 (sc-9034; Santa Cruz Biotechnology), XRCC6 (ALS14031; Abgent, San Diego, CA), TBX2 (H00006909-M01; Novus Biologicals), H3 (4499; Cell Signaling, Danvers, MA), DDB2 (Western blotting: 5416; Cell Signaling; ChIP: sc-25368; Santa Cruz Biotechnology), DNA-PKcs (NBP2-22128; Novus Biologicals), SEMA3A (AF1250; Novus Biologicals), actin (A4700; Sigma-Aldrich, St. Louis, MO), and y-H2AX (9718; Cell Signaling). DDB1 and CUL4A antibodies were described previously (Nag et al., 2004 (link)). Horseradish peroxidase–conjugated secondary antibodies were purchased from Bio-Rad.

Fantini D., Huang S., Asara J.M., Bagchi S, & Raychaudhuri P. (2017). Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2. Molecular Biology of the Cell, 28(1), 192-200.

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