The BAF complexes were immuno-purified from nuclear extract using anti-BRG1 or anti-BAF57 (for endogenous BAF) or anti-FLAG antibody (for FLAG-BRG1-containing BAF) and were spotted onto a nitrocellulose membrane (sample amount normalized by cell number). The membrane was allowed to dry at room temperature for 30 minutes. The membrane was rinsed with TBST (150mM NaCl, 20 mM Tris, pH7.4, 0.05% Tween-20), blocked with 5% BSA in TBST, and incubated with Biotin-XX Phalloidin (Biotium) either overnight at 4°C or for two hours at room temperature followed by three washes with TBST. The membrane was incubated with High Sensitivity Streptavidin-HRP (21130, Pierce/Thermo Fisher) for one hour and was washed three times with TBST, followed by detection with ECL reagent.
Biotin xx phalloidin
Manufactured by Biotium
Biotin-XX Phalloidin is a fluorescent dye that binds specifically to filamentous actin (F-actin) in cells. It is used as a tool for visualizing and labeling the actin cytoskeleton in various cell biology applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biotin xx phalloidin
1
Purification and Detection of BAF Complexes
2
Purification and Detection of BAF Complexes
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The BAF complexes were immuno-purified from nuclear extract using anti-BRG1 or anti-BAF57 (for endogenous BAF) or anti-FLAG antibody (for FLAG-BRG1-containing BAF) and were spotted onto a nitrocellulose membrane (sample amount normalized by cell number). The membrane was allowed to dry at room temperature for 30 minutes. The membrane was rinsed with TBST (150mM NaCl, 20 mM Tris, pH7.4, 0.05% Tween-20), blocked with 5% BSA in TBST, and incubated with Biotin-XX Phalloidin (Biotium) either overnight at 4°C or for two hours at room temperature followed by three washes with TBST. The membrane was incubated with High Sensitivity Streptavidin-HRP (21130, Pierce/Thermo Fisher) for one hour and was washed three times with TBST, followed by detection with ECL reagent.
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