The largest database of trusted experimental protocols

Anti mouse epcam pe

Manufactured by Thermo Fisher Scientific

Anti-mouse EpCAM-PE is a fluorescently-labeled antibody that binds to the epithelial cell adhesion molecule (EpCAM) expressed on mouse cells. This product is designed for use in flow cytometry applications to identify and quantify EpCAM-positive cells.

Automatically generated - may contain errors

2 protocols using anti mouse epcam pe

1

Isolation and Analysis of Ascites-Derived Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
10-13 week old REM2-KPf/fC mice were anesthetized and approximately 100μl of peripheral blood and ascites was collected in PBS containing 5mM EDTA and 2% Dextran. Samples were incubated at 37°C and red blood cells were lysed using RBC lysis buffer (eBiosciences). Remaining cells were stained with anti-mouse EpCAM-PE (eBiosciences) and anti-mouse CD45-PE-Cy7 (eBiosciences) antibodies. Analysis was carried out on a FACSAria III machine (Becton Dickinson) and data analyzed with FlowJo software (Tree Star).
+ Open protocol
+ Expand
2

Isolation and Analysis of Ascites-Derived Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
10-13 week old REM2-KPf/fC mice were anesthetized and approximately 100μl of peripheral blood and ascites was collected in PBS containing 5mM EDTA and 2% Dextran. Samples were incubated at 37°C and red blood cells were lysed using RBC lysis buffer (eBiosciences). Remaining cells were stained with anti-mouse EpCAM-PE (eBiosciences) and anti-mouse CD45-PE-Cy7 (eBiosciences) antibodies. Analysis was carried out on a FACSAria III machine (Becton Dickinson) and data analyzed with FlowJo software (Tree Star).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!