Fitc anti rat cd90 antibody

MSCs at passage 3 were digested by 0.25% trypsin, washed twice with PBS, and then used as a single cell suspension. Approximately 2.0 × 10⁵ cells were incubated with fluorescence-coupled antibodies for 30 min at 4 °C and analysed by flow cytometry (Calibur; BD Biosciences) with FlowJo 10. The antibodies used were as follows: FITC anti-rat CD90 antibody (Cat No. 328108, BioLegend, USA), FITC anti-CD105 antibody (Cat No. 323204, BioLegend, USA) and FITC anti-mouse CD146 antibody (Cat No. 134706, BioLegend, USA).

Passage 3 rat MSCs were digested by 0.25% trypsin, washed twice with PBS, and then resuspended in PBS as single cell suspension. The cells were incubated with fluorescence-coupled antibodies and isotype control antibodies, respectively (0.4 μg antibody: 1 × 10⁵ cells). The antibodies involved are as follows: PE anti-mouse/rat CD29 antibody (BioLegend, USA, catalog number: 102207), FITC anti-rat CD90 antibody (BioLegend, USA, catalog number: 206105), FITC mouse anti-rat CD44H (BD Biosciences, USA, catalog number: 550974), CD34 antibody-FITC (Santa Cruz Biotechnology, USA, catalog number: sc-7324 FITC), CD45 antibody-FITC (Santa Cruz Biotechnology, USA, catalog number: sc-1178 FITC), PE Armenian hamster IgG isotype Ctrl antibody (BioLegend, USA, catalog number: 400907), and FITC mouse IgG1, κ isotype Ctrl antibody (BioLegend, USA, catalog number: 400107). After 30 min of incubation at 4°C in the dark, rat MSCs were washed twice with PBS and resuspended in 300 μl PBS for analysis. Cell fluorescence was examined by a FACScan flow cytometer running CellQuest software.