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Mouse igg2aκ

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Mouse IgG2aκ is an immunoglobulin isotype found in mice. It is typically used as a control or reference antibody in various immunological applications.

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Lab products found in correlation

Anti human hla dr apc h7 clone g46 6, by Thermo Fisher Scientific (2 mentions) Anti human cd14 phycoerythrin pe cy7, by Thermo Fisher Scientific (2 mentions) Clone rpa t4, by Thermo Fisher Scientific (2 mentions) Clone rpa t8, by Thermo Fisher Scientific (1 mentions) Anti human cd127 fitc, by Thermo Fisher Scientific (1 mentions) Mouse igg2a k, by Thermo Fisher Scientific (1 mentions) Mouse igg1k, by Thermo Fisher Scientific (1 mentions) Anti human cd4 percp cy5, by BD (1 mentions) Clone m a251, by BD (1 mentions) Facsdiva, by BD (1 mentions)

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IgG2aκ (5 citations)

4 protocols using mouse igg2aκ

1

Multiparametric T Cell Phenotyping

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CD4+ and CD8+ T cells were enumerated by labeling with anti‐human CD4 PerCp/Cy5·5, mouse IgG1κ, clone RPA‐T4 from eBioscience and anti‐human CD8 AF700, mouse IgG1κ, clone RPA‐T8 from BD Bioscience. The activation state was defined by staining with anti‐human human leukocyte antigen D‐related (HLA‐DR) APC/H7, mouse IgG2aκ, clone G46‐6 and anti‐human IL‐7R FITC and mouse IgG1κ, clone eBioRDR5 from BD Bioscience. Regulatory T cells were enumerated and their functional phenotype determined by labeling with the following mAbs: CD25 BV421, mouse IgG1κ and clone M‐A251 from BD Bioscience, and anti‐human CD4 PerCp/Cy5.5, mouse IgG1κ, clone RPA‐T4, anti‐human IL‐7R FITC, mouse IgG1κ, clone eBioRDR5, anti‐human HLA‐DR APC/H7 clone G46‐6, mouse IgG2aκ from eBioscience.
Burns J.C., Hsieh L.E., Kumar J., Behnamfar N., Shimizu C., Sivilay N., Tremoulet A.H, & Franco A. (2020). Characterization of circulating immune cells in acute Kawasaki disease suggests exposure to different antigens. Clinical and Experimental Immunology, 202(3), 263-272.
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2

Immune Phenotyping of T Cells and Dendritic Cells in Kawasaki Disease

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Given the role of T cells and myeloid dendritic cells (DC) in the pro- and anti-inflammatory pathways in KD, we focused on immune phenotyping these cell populations. Myeloid DC populations were characterized and enumerated by flow cytometry using the following monoclonal antibodies (mAb) : anti-human CD11c-APC, mouse IgG1κ, clone B-ly6, anti-human CD11b-APC-Cyanin 7 (Cy7), mouse IgG1κ, clone ICRF44, anti-human CD14-phycoerythrin (PE) Cy7, mouse IgG2aκ, clone M5E2, anti-human CD86-fluorescein isothiocyanate (FITC), mouse IgG1κ, clone 2331 (FuN-1) (eBioscience).
T cell populations were characterized and enumerated by flow cytometry using the following mAb: CD25 BV421, mouse IgG1 k, clone M-A251 (BD Bioscience), anti-human CD4-percp-Cy5.5, mouse IgG1k, clone RPA-T4, anti-human CD8, Alexa Fluor 700, clone RPA-T8, mouse IgG1k, anti-human CD45RA APC, mouse IgG2b k, clone HI100, anti-human CD127 FITC, mouse IgG1 k, clone eBioRDR5, anti-human HLA-DR APC-H7 clone G46-6, mouse IgG2a k (eBioscience). Data were acquired with FACS ARIA II and analyzed using FACSDiva (BS Biosciences, San Jose, CA).
Tremoulet A.H., Jain S., Jone P.N., Best B.M., Duxbury E.H., Franco A., Printz B., Dominguez S.R., Heizer H., Anderson M.S., Glodé M.P., He F., Padilla R.L., Shimizu C., Bainto E., Pancheri J., Cohen H.J., Whitin J.C, & Burns J.C. (2019). Phase I/IIa Trial of Atorvastatin in Patients With Acute Kawasaki Disease With Coronary Artery Aneurysms. The Journal of pediatrics, 215, 107-117.e12.
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3

Neutrophil Functional Responses to CSF

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Adhesion molecule expression, phagocytosis, and reactive oxygen intermediate (ROI) production were measured in neutrophils isolated form HCs after incubation with CSF for 30 min in 37°C. Unconjugated anti-C5a (1 µg/mL, clone 2942; Thermo Fisher Scientific) and mouse IgG2aκ (eBioscience™) as isotype control mAbs were used for neutralizing experiments.
Piatek P., Domowicz M., Lewkowicz N., Przygodzka P., Matysiak M., Dzitko K, & Lewkowicz P. (2018). C5a-Preactivated Neutrophils Are Critical for Autoimmune-Induced Astrocyte Dysregulation in Neuromyelitis Optica Spectrum Disorder. Frontiers in Immunology, 9, 1694.
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4

Myeloid Dendritic Cell Immunophenotyping

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Myeloid dendritic cells (mDC) and macrophage populations and their maturation stage were defined by binding of the following monoclonal antibodies (mAb) to cell surface markers and analyzed by flow cytometry: anti‐human CD11c allophycocyanin (APC), mouse immunoglobulin (Ig)G1κ, clone B‐ly6, anti‐human CD11b APC/cyanin 7 (Cy7), mouse IgG1κ, clone ICRF44, anti‐human CD14 phycoerythrin (PE)/Cy7, mouse IgG2aκ, clone M5E2, anti‐human CD86 fluorescein isothiocyanate (FITC), mouse IgG1κ, clone 2331 (FuN‐1), anti‐human CD4 AF700 mouse IgG1κ, clone RPA‐T4, anti‐human ILT‐4 peridinin chlorophyll (PerCp)/eF710 and mouse IgG2bκ, clone 42D1 from eBioscience (San Diego, CA, USA). Data for all immunophenotyping were acquired with fluorescence activated cell sorter (FACS) ARIA II and analyzed using FACSDiva (BD Biosciences, San Jose, CA, USA) software. Normal pediatric ranges for mDC and tolerogenic mDC (tmDC) were previously determined by our group [5, 6].
Burns J.C., Hsieh L.E., Kumar J., Behnamfar N., Shimizu C., Sivilay N., Tremoulet A.H, & Franco A. (2020). Characterization of circulating immune cells in acute Kawasaki disease suggests exposure to different antigens. Clinical and Experimental Immunology, 202(3), 263-272.
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