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8 amino naphtalene 1 3 6 trisulfonic acid sodium salt ants

Manufactured by Thermo Fisher Scientific
Sourced in Germany

8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) is a fluorescent dye that can be used as a labeling agent. It has an excitation maximum at 356 nm and an emission maximum at 520 nm.

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2 protocols using 8 amino naphtalene 1 3 6 trisulfonic acid sodium salt ants

1

Synthesis and Labeling of Lipids

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15N-labelled amino acids were purchased from Cambridge Isotope Laboratories (Andover, MA, USA) and were Fmoc-protected using Fmoc-Cl as described previously [33 (link)]. The lipids 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), were purchased from NOF Europe (Grobbendonk, Belgium). The fluorescent lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) were obtained from Avanti Polar Lipids. The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and p-xylene-bis(pyridinium)bromide (DPX) were obtained from Invitrogen-Molecular Probes (Karlsruhe, Germany).
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2

Fluorescent Lipid Labeling Protocol

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Peptide synthesis reagents and Fmoc-protected amino acids were purchased from Iris Biotech (Marktredwitz, Germany) or Merck Biosciences (Darmstadt, Germany). Chemicals for peptide synthesis were obtained from Merck (Darmstadt, Germany) or Biosolve (Valkenswaard, Netherlands), and solvents for HPLC purification were purchased from Fischer Scientific (Geel, Belgium). 4-Chloro-7-nitrobenzofurazan (NBD-Cl) was purchased from VWR (Bruchsal, Germany). The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and p-xylene-bis-pyridinium bromide (DPX) were obtained from Invitrogen-Molecular Probes (Karlsruhe, Germany). Fluorescently labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) was obtained from Avanti Polar Lipids (Alabaster, AL, USA). The lipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), and 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG) were purchased from NOF (Grobbendonk, Belgium).
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