Hrp conjugated matched secondary antibodies

Western blots were performed using standard methods. Blots were incubated with primary antibodies commercially obtained against: anti-MUS81 (1:1000; Santa Cruz Biotechnologies), anti-SMARCAL1 (1:2000; Abcam), anti-RAD51 (1:5000; Abcam), anti-BrdU (1:50, Becton Dickinson), anti-BRCA2 (1:1000; Bethyl), anti-Lamin B1 (1:10000; Abcam), GFP (1:1000; Santa Cruz Biotechnology), GAPDH (1:5000; Millipore). HRP-conjugated matched secondary antibodies were from Jackson Immunoresearch and were used at 1:40 000.
Horseradish peroxidase-conjugated goat specie-specific secondary antibodies (Santa Cruz Biotechnology, Inc.) were used. Quantification was performed on scanned images of blots using ImageJ software, and values are shown on the graphs as indicated.

The primary antibodies used were: anti-MUS81 (1:1000; Santa Cruz Biotechnologies), anti-DDK (Flag Origene, WB 1:1000, IF 1:200), anti-cMYC (1:1000; Abcam), anti-CK2 (1:1000; Cell Signaling Technologies), anti-RxxpS/T (1:1000; Cell Signaling Technologies), anti-SLX4 (WB 1:1000, IF 1:200, Novus biologicals), anti-pS10H3 (1:1000, Santa Cruz Biotechnologies), anti-H3 (1:1000, Santa Cruz Biotechnologies), anti-EME1 (1:1000, Santa Cruz Biotechnologies), anti-EME2 (1:500, Invitrogen), anti-Cyclin A (WB: 1:1000, IF: 1:100, Santa Cruz Biotechnologies), anti 53BP1 (1:400, Millipore), anti-BrdU (1:50, Becton Dickinson), anti-pS139H2A.X (1:1000, Millipore), anti-γ-Tubulin (1:200, Sigma-Aldrich), anti-pS87MUS81 (WB 1:1000, IF 1:200, Abgent) and anti-Lamin B1 (1:10 000; Abcam). HRP-conjugated matched secondary antibodies were from Jackson Immunoresearch and were used at 1:40 000.