Analysis of optical properties of CDs was performed using PerkinElmer LS 45 fluorescence spectrophotometer. UV-Vis absorption spectra were recorded on PerkinElmer lamda 2 UV-visible spectrophotometer. The identification of functional groups was done using a Bomem FT-IR. Carbon, hydrogen, and nitrogen contents were determined by a Vario EL III elementar instrument (Elmentar Analysensysteme GmbH). Horiba SZ-100 zeta potential analyzer was used for zeta potential measurements. Particle size distribution of CDs was characterized by DLS (Nanophox, Sympatec GmbH). The shape and surface morphology of CDs nanoparticles were evaluated by atomic force microscopy (AFM). Of the CDs solution, 1 μL was deposited onto a freshly cleaved mica surface and dried in air. The AFM instrument was used to capture the morphology of CDs. AFM imaging of samples was conducted on a DME instrument (Dualscope C-26, with DME-SPM v2.1.1.2 software) by tapping-mode in air under ambient conditions.
Sz 100 zeta potential analyzer
Manufactured by Horiba
The SZ-100 is a zeta potential analyzer manufactured by Horiba. It measures the zeta potential of particles or molecules in liquid dispersions. The zeta potential is a key parameter that indicates the stability and surface charge of colloidal systems. The SZ-100 utilizes the technique of electrophoretic light scattering to determine the zeta potential of samples.
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Lab products found in correlation
3 protocols using sz 100 zeta potential analyzer
1
Optical and Physicochemical Characterization of Carbon Dots
2
Zeta Potential Analysis of GK-16 Solution
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HORIBA SZ-100 Zeta potential analyzer was utilized to measure the zeta potential of GK-16* solution (1 mg/mL) under different pH values based on laser Doppler electrophoresis. Zeta potential glass cell of 100 μL was chosen with measurement angle of 90°. n = 3 biologically independent samples over 3 independent experiments. Data are presented as mean values ± SD.
3
Characterization of Synthesized AuNPs
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The SPR spectra were acquired at room temperature using a UV-Vis spectrophotometer directly after synthesis using a 1 cm optical path length quartz cuvette and 200-800 nm wavelength range. The morphologies of the AuNPs were characterized by Transmission Electron microscopy (TEM, JEOL JEM-1400). AuNPs for TEM analysis were prepared by immersing the copper grid into AuNPs solution then drying at room temperature. AuNPs size was determined by Particle Size Analysis (PSA, Horiba SZ-100). The zeta potential of the AuNPs solution was assessed using a Horiba SZ-100 Zeta Potential Analyzer. The Particle size and zeta potential were directly measured after synthesis using a 1 cm optical path length cuvette. The crystallinity of AuNPs achieved was investigated by Shimadzu XRD 6000. The interaction between AuNPs, reducing agent, capping agent, and Hg 2+ was analyzed by FTIR. The sample was prepared by collecting nanoparticle deposits from the centrifugation of the nanoparticle solution.
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