Solvents used were of analytical or HPLC grade and were purchased from Merck (Darmstadt, Germany). The 1H and 13C NMR analyses were run on a Bruker AMX-500 NMR spectrometer (Karlsruh, Germany), operating at 400 MHz for 1H and 125 MHz for 13C NMR, respectively. DMSO-d6 as solvent and tetramethyl silane (TMS) as internal standard were used. HR-ESIMS analysis was carried out with an LCT Premier XE Micromass Waters spectrometer in the positive and negative ionization modes (Waters Corporation). Detection of compounds was carried out on precoated silica gel 60 F254 plates (0.25 mm layer, E. Merck, Darmstadt, Germany), using chloroform–methanol–water (8:2:0.2) as a mobile phase, and visualized with 5% H2SO4 spray reagent after heating at 110 °C.
Lct premier xe micromass waters spectrometer
Manufactured by Waters Corporation
The LCT Premier XE Micromass Waters spectrometer is a laboratory instrument designed for high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. The core function of this product is to provide accurate and sensitive mass spectrometric detection and identification of a wide range of organic compounds in complex samples.
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2 protocols using lct premier xe micromass waters spectrometer
1
NMR and Mass Spectrometry Analysis Protocol
2
Spectroscopic Analysis of Organic Compounds
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NMR spectra were obtained by Bruker AMX-500 spectrometer with standard pulse sequences, operating at 500 MHz for 1 H and 125 MHz for 13 C. MeOH-d4, was used as solvent and TMS as internal standard. HR-ESIMS was performed with a LCT Premier XE Micromass Waters spectrometer in positive ionization mode (Waters Corporation). Column chromatography (CC) was carried out with silica gel Fluka (cat. 60737) (40-63 μm), and column fractions were monitored by thin layer chromatography (TLC) silica gel 60 F254, 0.2 mm, Macherey Nagel (cat. 818-333) by detection with a spraying reagent (CH3COOH/H2O/H2SO4; 80:16:4) followed by heating at 100 °C. Preparative TLC was carried out on silica gel 60 PF254 + 366 (20 × 20 cm, 1 mm thickness, Analtech cat. 02014).
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