6 13c d glucose

Deuterium oxide, ¹⁵N-ammonium chloride, 6-¹³C-D-glucose, alpha-ketobutyric acid [methyl-¹³C;3,3-D₂], alpha-ketoisovaleric acid [3-methyl-¹³C; 3,4,4,4-D₄] was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). Sodium tungstate dihydrate was purchased from MP Biomedicals. p-Nitrophenyl phosphate disodium salt was purchased from Santa Cruz Biotechnology. All oligonucleotide sequences were purchased from the Keck Biotechnology Resource Laboratory (Yale University). Confirmation of the desired DNA sequences was confirmed by DNA sequencing (Keck Biotechnology Resource Laboratory, Yale University).

Deuterium oxide, ¹⁵N-Ammonium Chloride and 6-¹³C-D-Glucose was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). Sodium Vanadate was purchased from Fischer Scientific (Fair Lawn, NJ). p-Nitrophenol phosphate disodium salt was purchased from Santa Cruz Biotechnology (Dallas, TX).
The Escherichia coli codon-optimized MptpA Wild Type plasmid was purchased from GenScript (Piscataway, NJ). The gene contained a sequence upstream of the MptpA DNA sequence that adds a His₆ tag at the N-terminal and a TEV cleavage site (ENLYFQ/S). The gene was cloned into a pET-30a(+) vector using the NdeI and BamHI restriction sites.