Hispidulin (ChemFaces, Wuhan, China), tween 80 (Srichansahasoth Co., Thailand), acetic acid (Merck, Darmstadt, Germany), formaldehyde (Merck), indomethacin (IND; Sigma), morphine sulfate (MRP; Thai FDA), and sterile water (SW; General Hospital Products Public Co.) were used in this study. HPLC grade acetonitrile and methanol (MeOH) were purchased from RCI Labscan (Bangkok, Thailand). Ultrapure water was obtained from Barnstead™ MicroPure™ Water Purification System (Thermo Fisher Scientific, Massachusetts, USA). Glacial acetic acid was purchased from Merck KGaA (Darmstadt, Germany).
Barnstead micropure water purification system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Barnstead™ MicroPure™ Water Purification System is a laboratory water purification device. It produces high-purity deionized water by using a combination of filtration and purification technologies.
Automatically generated - may contain errors
Lab products found in correlation
Sulfuric acid, by Thermo Fisher Scientific (2 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)
Ferric chloride hexahydrate, by Thermo Fisher Scientific (2 mentions)
Ammonium dihydrogen phosphate, by Thermo Fisher Scientific (2 mentions)
Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (2 mentions)
Malic acid, by Merck Group (2 mentions)
Orthophosphoric acid, by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (2 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (2 mentions)
L tartaric acid, by Thermo Fisher Scientific (2 mentions)
0.1 n sodium hydroxide, by Thermo Fisher Scientific (2 mentions)
Potassium metabisulfite, by Thermo Fisher Scientific (2 mentions)
Malvidin 3 o glucoside chloride oenin, by Extrasynthese (2 mentions)
Malvidin 3 5 o diglucoside chloride malvin, by Extrasynthese (2 mentions)
D fructose, by Thermo Fisher Scientific (2 mentions)
Quercetin 3 glucoside, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Catechin, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
5 protocols using barnstead micropure water purification system
1
Hispidulin-Based Pain Management Protocol
2
Comprehensive Analytical Protocol for Phytochemicals
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Hydrochloric acid, sodium hydroxide, glacial acetic acid, ferric chloride hexahydrate, ortho-phosphoric acid (≥85%), acetonitrile, sulfuric acid, L-(+)-tartaric acid, D-fructose, and 0.1 N sodium hydroxide were purchased from Fisher Scientific (Santa Clara, CA, USA). Sodium chloride, bovine serum albumin (BSA), (-)-epicatechin (≥90%), (+)-catechin (≥98%), caffeic acid (≥98%), malic acid (≥99%), and quercetin-3-glucoside (≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium metabisulfite (≥97%), sodium dodecyl sulfate, and ammonium dihydrogen phosphate (>98%) were purchased from Acros Organics (Geel, Belgium). Triethanolamine was purchased from Aqua Solutions, Inc. (Deer Park, TX, USA). Commercial malvidin-3-O-glucoside chloride (oenin) (≥95%) and malvidin-3,5-O-diglucoside chloride (malvin) (≥95%) were provided by Extrasynthese (Genay, France). Milli-Q water used for the preparation of solutions was obtained from a Barnstead MicroPure Water Purification System (Thermo scientific®, Waltham, MA, USA).
3
Analytical Characterization of Food Bioactive Compounds
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Acetonitrile, D-fructose, ferric chloride hexahydrate, glacial acetic acid, hydrochloric acid, L-(+)-tartaric acid, ortho-phosphoric acid (≥85%), sodium hydroxide, sulfuric acid, and 0.1 N sodium hydroxide were purchased from Fisher Scientific (Santa Clara, CA, USA). Bovine serum albumin (BSA), caffeic acid (≥98%), (+)-catechin (≥98%), (−)-epicatechin (≥ 90%), malic acid (≥99%), quercetin-3-glucoside (≥98%), and sodium chloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium dihydrogen phosphate (>98%), potassium metabisulfite (≥97%), and sodium dodecyl sulfate were purchased from Acros Organics (Geel, Belgium). Commercial malvidin-3-O-glucoside chloride (oenin) (≥95%) and malvidin-3,5-O-diglucoside chloride (malvin) (≥95%) were provided by Extrasynthese (Genay, France). Triethanolamine was purchased from Aqua Solutions, Inc. (Deer Park, TX, USA). Milli-Q grade water (18.2 MΩ cm resistivity) used for the preparation of reagent, solutions, and high-performance liquid chromatography with diode array detector (HPLC-DAD) mobile phase was obtained from Barnstead MicroPure Water Purification System (Thermo scientific®, Waltham, MA, USA).
4
Antioxidant and Antimicrobial Characterization
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High-performance thin-layer chromatography (HPTLC) silica gel 60 F254 glass plates, 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu (FC) reagent, gallic acid, ascorbic acid, hydrochloric acid (HCl), formic acid, reagent grade butanol (BuOH), absolute ethanol (EtOH), diphenylborinic acid aminoethyl ester, polyethylene glycol 400, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), iron (III) chloride hexahydrate (FeCl3·6H2O), iron (II) sulfate heptahydrate (FeSO4·7H2O), disodium ethylenediaminetetraacetate (Na2-EDTA), sodium carbonate (Na2CO3), nitroblue tetrazolium (NBT), riboflavin, Trolox, quercetin, gentamicin, and amphotericin B were purchased from Sigma-Aldrich (Burlington, MA, USA) and Merck KGaA (Darmstadt, Germany). Mueller–Hinton Broth, Mueller–Hinton agar, and Sabouraud dextrose agar were purchased from Difco (BD Diagnostics, Sparks, MD, USA). DMSO-d6 was acquired from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Deionized water was generated with a Barnstead™ MicroPure™ Water Purification System (Thermo Fisher Scientific, Waltham, MA, USA). Commercial-grade hexanes, dichloromethane, ethyl acetate (EtOAc), methanol (MeOH), and 95% EtOH were acquired from T.S. Interlab LP (Bangkok, Thailand) and distilled before use.
5
Synthesis and Characterization of P(TEGA-co-SPA) Copolymer
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P (TEGA-co-SPA) synthesis was reproduced from reference [45 (link)]. More details are provided in the Supplementary Section. Using this method, spiropyran acrylate (SPA) was obtained as a yellow powder. Commercial TEGA monomer was copolymerized with 15 mol% SPA in the initial monomer mixture. The obtained copolymer was investigated via size exclusion chromatography with triple detection to obtain absolute molar masses and 1H liquid NMR to determine the composition by comparing the signal of the SPA moiety (8.2 ppm, 2H) and the TEGA moiety (3.3 ppm, 3H). The fraction of SPA in the obtained copolymer was between 12–14 mol%, the molar mass Mn was about 33,000 g/mol with a dispersity index PDI = 1.7. An aqueous solution of 0.15 wt.% P (TEGA-co-SPA) was obtained by diluting the copolymer in deionized water. Deionization was done using a Thermo Scientific Barnstead MicroPure water purification system to a resistivity of 18.2 MΩ cm−1.
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