The optical absorbances of pristine CDNA and S/D/T-CDNA NLs were measured using a Cary 5G spectrophotometer (Varian, CA, USA). The spectrophotometer was equipped with two light sources (a deuterium arc lamp and a quartz W – halogen lamp) and two detectors (a cooled PbS detector and a photomultiplier tube) (Fig. 5 and 6 ).
Cary 5g spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States
The Cary 5G spectrophotometer is a high-performance UV-Vis-NIR instrument designed for accurate and reliable measurements across a wide wavelength range. It features a compact, modular design and advanced optics to deliver precise absorbance, reflectance, and transmittance data.
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3 protocols using cary 5g spectrophotometer
1
Optical Absorbance Measurement of CDNA NLs
2
Optical Transmission of DOX-doped DNA Films
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A Varian Cary 5G spectrophotometer was used to conduct the optical transmission measurements of the DOX−doped DNA thin films on quartz in the near-infrared, visible, and ultraviolet regions (wavelengths between 1200 and 190 nm). The spectrophotometer was equipped with two light sources: a deuterium arc lamp (near-infrared and visible) and a quartz W−halogen lamp (ultraviolet). It also has two detectors: a cooled PbS detector for the near-infrared region and a photomultiplier tube for the visible and ultraviolet regions. The spectrophotometer measured the frequency-dependent light intensity passing either through a vacuum or through the sample.
3
Optical Absorbance of DNA-Doxorubicin Complexes
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A Varian Cary 5G spectrophotometer was used to conduct the optical absorbance measurements of the DNA, DNA with doxorubicin, Cu2+-DNA, and Cu2+-DNA with doxorubicin in solution form (wavelengths between 800 and 200 nm). The spectrophotometer was equipped with two light sources: a deuterium arc lamp (near-infrared and visible) and a quartz W− halogen lamp (ultraviolet). It has also two detectors: a cooled PbS detector for the near-infrared region and a photomultiplier tube for the visible and ultraviolet regions. The spectrophotometer measure the frequency-dependent light intensity passing either through a vacuum or through the sample.
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