Cells were transfected with 20 nM of Myo5A predesigned siRNA (SASI_Hs01_00181251; Sigma Aldrich), Myo5B predesigned siRNA (SASI_Hs01_00220872; Sigma Aldrich), RAB11A predesigned siRNA (SASI_Hs01_00126206; Sigma Aldrich), RAB11B predesigned siRNA (SASI_Hs01_00220872; Sigma Aldrich) or Negative control siRNA (SIC002; Sigma Aldrich) for 5 h at 37 °C in SFM using Lipofectamine RNAiMAX (Thermo Fisher Scientific), then were maintained in LHC8 complete medium and used in the following experiments.
+ Open protocol