Methylation of fatty acids present in oil samples was carried out as described elsewhere [41 ]. Analysis of FAMEs was performed by using an Agilent 7820A GC System (Palo Alto, CA, USA) equipped with a FID detector. A TR-CN100 column (60 m × 0.25 mm × 0.2 µm; Teknokroma, Barcelona, Spain) was used. The GC oven was programmed from 120 to 250 °C (hold 15 min) at 5 °C min−1. Helium was used as carrier gas (1 mL min−1) and 1 µL was injected in the split mode (1:75). The tridecanoic acid methyl ester (700 mg kg−1) was used as internal standard whereas analytical standards were used for external calibration.
Tr cn100 column
Manufactured by Teknokroma
Sourced in Spain, Japan
The TR-CN100 column is a laboratory equipment designed for chromatographic separation. It features a stainless steel construction and is suitable for a variety of applications. The core function of the TR-CN100 is to facilitate the separation and purification of chemical compounds.
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Lab products found in correlation
2 protocols using tr cn100 column
1
Fatty Acid Profiling in Oil Samples
2
Lipid Extraction and Fatty Acid Profiling
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Total lipids were extracted by the method of Bligh and Dyer (1959) (link). Fatty acid composition was determined after methylation (ISO, 1978 ) by GC-MS (Shimadzu model of QP2010 Plus, Shimadzu Corporation, Japan) using a Teknokroma TR-CN 100 column (60 m × 0.25 mm I.D., 0.20 μm) (Teknokroma, Spain). The temperature of the injector port and detector was held at 250℃. The injected volume was 1.0 μl. The carrier gas was helium at a pressure of 200 kPa. The split used was 1:100. The temperature of the column was held at 90℃ for 7 min, raised to 240℃ at 5℃/min and finally held at 240℃ for 10 min. Fatty acids were identified by comparison of their retention times with those of authentic standards (Supelco 37 Components FAME Mixture, Cat. No. 18919-1AMP, USA) and reported as the percentage of total fatty acids determined.
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