Dihydrotestosterone dht

Manufactured by Selleck Chemicals

Dihydrotestosterone (DHT) is a naturally occurring androgen hormone. It is the primary active metabolite of the hormone testosterone. DHT plays a crucial role in the development and maintenance of male sexual characteristics.

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Lab products found in correlation

Progesterone p4, by Merck Group (1 mentions) β oestradiol e2, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Nutlin 3, by Selleck Chemicals (1 mentions) Arn 509, by Selleck Chemicals (1 mentions) R1881, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions)

2 protocols using dihydrotestosterone dht

Steroid Hormone Regulation in T47D Cells

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5×10⁵ T47D cells were plated in 6-well dishes and cultured in RPMI-1640 and 10% HI-FBS for 24 h. The next day, the cells were washed with 1× PBS and the media was replaced with RPMI-1640 (phenol red free) (Thermo Fisher) and 10% Dextran Coated Charcoal stripped (DCC) FBS for 48 h prior to steroid treatment. The cells were treated with either 10 nM β-oestradiol (E2) (Sigma), 10 nM progesterone (P4) (Sigma), 10 nM dihydrotestosterone (DHT) (Selleck Chemicals) or vehicle (100% EtOH) for the indicated times and then harvested. Cells were stored at −80 °C until analysed.

de Nys R., van Eyk C.L., Ritchie T., Møller R.S., Scheffer I.E., Marini C., Bhattacharjee R., Kumar R, & Gecz J. (2024). Multiomic analysis implicates nuclear hormone receptor signalling in clustering epilepsy. Translational Psychiatry, 14, 65.

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Prostate Cancer Cell Culture and Inhibitor Assays

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LNCaP, PC3 and 22RV1 cells (Chinese Academy of Sciences) were cultured using RPMI1640 containing 10% serum. Myc-CaP cells were obtained from Shanghai Jiao Tong University (Shanghai, China). HEK293T was provided by the Stem Cell Bank and cultured using DMEM containing 10% serum. Androgen deprivation was conducted by culturing cells in RPMI1640 (5% charcoal-stripped serum) for 48 h.
ENZ (Cat# HY-70002), actinomycin D (ACTD) (Cat# HY-17559) and cycloheximide (CHX Cat# 66-81-9) were purchased from MCE. ARN-509 (Cat# 956104-40-8), ODM-201 (Cat# 1297538-32-9), MG132 (Cat# 1211877-36-9), dihydrotestosterone (DHT, Cat# 521-18-6), Nutlin3 (Cat# 890090-75-2) and R1881(Cat# 965-93-5) were obtained from Selleck. Recombinant Human MDM2/HDM2 Protein was obtained from R&D.

Li Y., Chu Y., Shi G., Wang X., Ye W., Shan C., Wang D., Zhang D., He W., Jiang J., Ma S., Han Y., Zhao Z., Du S., Chen Z., Li Z., Yang Y., Wang C., Xu X, & Wu H. (2022). A novel inhibitor of ARfl and ARv7 induces protein degradation to overcome enzalutamide resistance in advanced prostate cancer. Acta Pharmaceutica Sinica. B, 12(11), 4165-4179.

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