The aerial parts (leaves, stems, and flowers) of H. shizopetalus were collected during the flowering stage in May 2019, from Orman Botanic Garden, Giza, Egypt and authenticated by Mrs. Therese Labib, Botanical specialist and consultant at Orman and Qubba Botanic Gardens. A voucher specimen (26 November 2019) was deposited in the Museum of the Department of Pharmacognosy, College of Pharmacy, Cairo University, Egypt.
We used analytical grade solvents (ethanol, dichloromethane, and n-butanol) for plant extraction and fractionation (El-Gomhouria Co., Cairo, Egypt).
The chemicals 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ascorbic acid, and trichloro acetic acid were obtained from Aldrich Chemicals, U.S.A. Sodium phosphate, ammonium molybdate, potassium persulfate, potassium ferricyanide, and ferric chloride were purchased from El-Nasr Company for Pharmaceutical Chemicals, Egypt. Phosphate buffer was purchased from Bio diagnostic, Egypt. HPLC-grade Acetonitrile and methanol were purchased from SDFCL Fine-Chem Limited, Mumbai, India.
We used analytical grade solvents (ethanol, dichloromethane, and n-butanol) for plant extraction and fractionation (El-Gomhouria Co., Cairo, Egypt).
The chemicals 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ascorbic acid, and trichloro acetic acid were obtained from Aldrich Chemicals, U.S.A. Sodium phosphate, ammonium molybdate, potassium persulfate, potassium ferricyanide, and ferric chloride were purchased from El-Nasr Company for Pharmaceutical Chemicals, Egypt. Phosphate buffer was purchased from Bio diagnostic, Egypt. HPLC-grade Acetonitrile and methanol were purchased from SDFCL Fine-Chem Limited, Mumbai, India.