After collection, the blood was allowed to clot by leaving it undisturbed at room temperature for 1 h, and the supernatant was collected by centrifugation at 3500 rpm for 10 min. Serum levels of pro-inflammatory cytokine was measured using the Bio Legend LEGEND plex™ multiplex bead-based assay (#740007) from Bio Legend (San Diego, CA, USA).
Legend plex multiplex bead based assay
Manufactured by BioLegend
Sourced in United States
LEGEND plex™ is a multiplex bead-based assay system. It allows for the simultaneous detection and quantification of multiple analytes in a single sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using legend plex multiplex bead based assay
1
Serum Cytokine Profiling Protocol
2
Multiplex Cytokine Quantification
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Cytokines were quantified using Legendplex multiplex bead-based assay (Biolegend) using the mouse anti-virus response panel according to manufacturer’s instructions. Flow cytometry of the beads was performed using a FACSAria Fusion and data were analyzed using Biolegend’s cloud-based analysis software available at https://legendplex.qognit.com .
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