Nigericin and cisplatin were obtained from Sigma-Aldrich; Merck KGaA. Nigericin (25 mM stock) was dissolved in DMSO. cisplatin (1 mM stock) was dissolved in normal saline (0.9% NaCl). Both stock solutions were stored as aliquots at −20°C. DMSO served as a vehicle control. The following primary antibodies were used at the dilution recommended by the manufacturer (1:1,000) and purchased from Cell Signaling Technology, Inc.: Rabbit anti-Axin-2 (cat. no. 2151), rabbit anti-MMP7 (cat. no. 71031), rabbit anti-vimentin (cat. no. 5741), rabbit anti-tubulin (cat. no. 2148), rabbit anti-β-catenin (cat. no. 25362), rabbit anti-slug (cat. no. 9585), rabbit anti-E-cadherin (cat. no. 3195) and rabbit anti-small ubiquitin-like modifier 1 (SUMO1; cat. no. 4930). Mouse anti-GSK3β (cat. no. sc-377213) was used at a 1:200 dilution, and purchased from Santa Cruz Biotechnology, Inc. Goat anti-mouse IgG (HRP) (ZB-2305) and Goad anti-rabbit IgG (HRP) (ZB-2301) were used at a dilution of 1:2,500, and were purchased from ZSGB-BIO, Inc. (http://www.zsbio.com/ ).
Rabbit anti axin 2
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-Axin-2 is a primary antibody that recognizes the Axin-2 protein. Axin-2 is a key regulator of the Wnt signaling pathway, which is involved in various cellular processes such as cell fate determination, cell proliferation, and embryonic development. The antibody is raised in rabbits and can be used to detect and study the expression and localization of Axin-2 in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Nigericin, by Merck Group (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Cisplatin, by Merck Group (1 mentions)
Goat anti mouse igg hrp zb2305, by ZSGB-BIO (1 mentions)
Active β catenin, by Cell Signaling Technology (1 mentions)
Mouse anti cyclin d1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti tubulin, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Protease inhibitor, by Thermo Fisher Scientific (1 mentions)
Mouse anti tubulin, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
3 protocols using rabbit anti axin 2
1
Investigating Nigericin and Cisplatin Effects
2
Wnt Signaling Pathway Protein Expression
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The following antibodies were used: rabbit anti-non-phospho (Active) β-catenin (Cell signaling, 19807, 1:2000), mouse anti-tubulin (Sigma, T-9026, 1:10,000), mouse anti-Cyclin-D1 (Santa Cruz Biotechnology sc-20044, 1:500), mouse anti-DHX29 (Santa Cruz Biotechnology sc-81080, 1:200), rabbit anti-Ki-67 (Abcam, ab15580WB, immunofluorescence 1:250), mouse anti-APC (Calbiochem, OP44), rabbit anti-Axin2 (Cell Signaling, 76G6, 1:1000). The secondary antibodies used were HRP anti-mouse and anti-rabbit (Jackson Immuno Research, 1:10 000) or Alexa fluor 488 goat anti-rabbit 1:500 (Invitrogen, A11034).
3
Western Blot Analysis of Wnt Signaling
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The collected cells were lysed in RIPA buffer (Thermo Scientific) with protease inhibitors (Thermo Scientific) after rinsing twice with PBS (precooled at 4 °C). Proteins were quantified in the bicinchoninic acid assay (Pierce BCA Protein Assay, Rockford, IL, USA), and then separated by SDS–polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene fluoride membranes. After blocking in 5% nonfat milk 1 h at 25 °C, the membranes were probed with primary antibodies followed by horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA). Primary antibodies used were rabbit anti-active β-Catenin protein (1:1,000, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-AXIN2 (1:1,000, Cell Signaling Technology) and mouse anti-Tubulin (1:1,000, Cell Signaling Technology). Immunoreactive bands were visualized with horseradish peroxidase substrate (Luminata, Millipore, Billerica, MA, USA) using the ChemiDocTM XRS+ system (Bio-Rad, Hercules, CA, USA).
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