Super dhb 9 1 mixture of 2 5 dihydroxybenzoic acid

Manufactured by Merck Group

Sourced in Germany

Super-DHB is a 9:1 mixture of 2,5-dihydroxybenzoic acid, a common matrix compound used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

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2 protocols using super dhb 9 1 mixture of 2 5 dihydroxybenzoic acid

Peptide-N-Glycosidase F Protocol

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Nonidet P-40 substitute (NP-40), potassium dihydrogenphosphate (KH₂PO₄), disodium hydrogen phosphate dihydrate (Na₂HPO₄ × 2 H₂O), sodium chloride (NaCl), 50 % sodium hydroxide (NaOH), 1-hydroxybenzotriazole 97 % (HOBt) and super-DHB (9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid, sDHB) were obtained from Sigma-Aldrich (Steinheim, Germany). Potassium hydroxide (KOH), sodium dodecyl sulfate (SDS), analytical grade ethanol and trifluoroacetic acid (TFA) were purchased from Merck (Darmstadt, Germany). HPLC-grade acetonitrile (ACN) was purchased from Biosolve (Valkenswaard, The Netherlands) and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) hydrochloride was obtained from Fluorochem (Hadfield, UK). Recombinant peptide-N-glycosidase F (PNGase F) was purchased from Roche Diagnostics (Mannheim, Germany). From a Millipore Q-Gard 2 system (Amsterdam, The Netherlands) maintained at ≥ 18 MΩ milli-Q water (MQ) was generated.

Vreeker G.C., Vangangelt K.M., Bladergroen M.R., Nicolardi S., Mesker W.E., Wuhrer M., van der Burgt Y.E, & Tollenaar R.A. (2021). Serum N-glycan profiles differ for various breast cancer subtypes. Glycoconjugate Journal, 38(3), 387-395.

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Optimized Carbohydrate Analysis Protocol

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Analytical grade ethanol,
sodium dodecyl sulfate (SDS), trifluoroacetic acid (TFA), and potassium
hydroxide (KOH) were obtained from Merck (Darmstadt, Germany). Disodium
hydrogen phosphate dihydrate (Na₂HPO₄ ×
2H₂O), potassium dihydrogen phosphate (KH₂PO₄), sodium chloride (NaCl), N,N′-diisopropylcarbodiimide (DIC), N,N′-dicyclohexylcarbodiimide (DCC), 85% phosphoric
acid (H₃PO₄), 50% sodium hydroxide (NaOH), nonidet
P-40 substitute (NP-40), 1-hydroxybenzotriazole 97% (HOBt) and super-DHB
(9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic
acid, sDHB) were purchased from Sigma-Aldrich (Steinheim, Germany).
1-Ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) hydrochloride
was obtained from Fluorochem (Hadfield, UK), whereas recombinant peptide-N-glycosidase F (PNGase F) was obtained from Roche Diagnostics
(Mannheim, Germany) and HPLC-grade acetonitrile (ACN) was purchased
from Biosolve (Valkenswaard, The Netherlands). Milli-Q water (MQ)
was generated from a Q-Gard 2 system (Millipore, Amsterdam, The Netherlands),
which was maintained at ≥18 MΩ.

Vreeker G.C., Nicolardi S., Bladergroen M.R., van der Plas C.J., Mesker W.E., Tollenaar R.A., van der Burgt Y.E, & Wuhrer M. (2018). Automated Plasma Glycomics with Linkage-Specific Sialic Acid Esterification and Ultrahigh Resolution MS. Analytical Chemistry, 90(20), 11955-11961.

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