Human hepatocellular carcinoma(HCC) HepG2, Hep 3B,SK-Hep1 and normal hepatocytes Chang cells were obtained from American Type Culture Collection (ATCC). AMPK α WT and KO mouse embryonic fibroblast (MEF) cells were obtained from Dr. Joohuh Ha. HepG2, SK-Hep1, Chang and MEF cells were cultured in DMEM supplemented with 10% FBS and 1% antibiotic (Welgene, South Korea).
Sk hep1
Manufactured by Welgene
Sourced in Cameroon
SK-Hep1 is a human liver adenocarcinoma cell line. It is a widely used in vitro model for the study of liver cancer biology.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Welgene (3 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (3 mentions)
Rpmi 1640, by Welgene (2 mentions)
Hepg2, by American Type Culture Collection (2 mentions)
Sk hep1, by American Type Culture Collection (2 mentions)
An3ca, by American Type Culture Collection (1 mentions)
Hep3b, by Welgene (1 mentions)
An3ca, by Welgene (1 mentions)
Mda mb 453, by Welgene (1 mentions)
Antibiotic antimycotic solution, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Snu 449, by American Type Culture Collection (1 mentions)
Plc prf 5, by American Type Culture Collection (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Plc prf 5, by Welgene (1 mentions)
Snu449, by Welgene (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
3 protocols using sk hep1
1
Cultivation of Hepatocellular Carcinoma Cells
2
Cell Culture Protocol for Cancer Research
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AN3-CA and J82 cells were obtained from ATCC (Manassas, VA, USA). KMS-11 cells were purchased from JCRB. HEP3B, HUH7, HEPG2, MDA-MB-453 and SK-HEP1 cells were purchased from KCLB (Seoul, Korea). HEP3B, AN3-CA, MDA-MB-453 and SK-HEP1 cells were cultured in DMEM (#LM001-05, Welgene). HUH7, HEPG2, J82 and KMS-11 cells were cultured in RPMI1640 (#LM011-01, Welgene). The culture media were supplemented with 10% fetal bovine serum (#S001-01, Welgene), antibiotic-antimycotic solution (#LS203-01, Welgene) containing 10,000U/mL penicillin. The cells were maintained in a humidified atmosphere containing 5% CO2 at 37 °C.
3
Culturing HCC Cell Lines
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HCC cell lines HepG2, SK-Hep-1, SNU449, and PLC/PRF/5 were purchased from the ATCC. HepG2 and PLC/PRF/5 cells were cultured in Minimum Essential Medium Eagle (MEM; WelGene, Daegu, Korea). SK-Hep-1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; WelGene). SNU449 cells were cultured in Roswell Park MEMorial Institute Medium 1640 (RPMI-1640; WelGene). All the cell lines, supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, New York, USA) and 1% penicillin/streptomycin (WelGene) were incubated at 37 °C in a 5% CO2 incubator.
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