The siRNA for WDFY3-AS2 (si-WDFY3-AS2) or PTEN (si-PTEN) was used to knock down WDFY3-AS2 or PTEN. WDFY3-AS2 plasmid was subcloned into the pcDNA3.1 vector to overexpress WDFY3-AS2 (pcDNA3.1-WDFY3-AS2). MiR-18a mimic, miR-18a inhibitor, and corresponding negative controls (NC-mimic and NC-inhibitor) were purchased from GenePharma Company (Shanghai, China). The transfections were performed using by Lipofectamine 2000 reagent (Invitrogen) following the instructions of the manufacturer.
Mir 18a inhibitor
Manufactured by GenePharma
Sourced in China
The MiR-18a inhibitor is a laboratory product designed to inhibit the activity of the microRNA (miRNA) molecule miR-18a. miR-18a is a small, non-coding RNA that plays a regulatory role in gene expression. The inhibitor functions by directly binding to and neutralizing miR-18a, thereby modulating the biological processes in which miR-18a is involved.
Automatically generated - may contain errors
Lab products found in correlation
Mir 18a mimic, by GenePharma (3 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Mimic nc, by GenePharma (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Mimic control, by GenePharma (1 mentions)
Inhibitor control, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Mir 21 mimic, by GenePharma (1 mentions)
Mir 21 inhibitor, by GenePharma (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Huvecs, by National Collection of Authenticated Cell Cultures (1 mentions)
3 protocols using mir 18a inhibitor
1
Knockdown and Overexpression of WDFY3-AS2 and PTEN
2
Overexpression and Inhibition of miRNAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The transfection was performed using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer. The transfected cells were cultured for 48 h before analysis. The CASC2 overexpression vector (pcDNA3.1-CASC2), empty vector (pcDNA3.1), miR-18a inhibitor, miR-21 inhibitor, inhibitor control, miR-18a mimics, miR-21 mimics and mimic control were synthesized by Shanghai GenePharma Co., Ltd. (Shanghai, China).
3
Evaluating miR-18a Modulation in HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HUVECs were obtained from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultivated in Dulbecco's Modified Eagle Medium (Life Technologies/Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum (Invitrogen/Thermo Fisher Scientific, Waltham, MA) and incubated at 37°C in a 5% CO 2 humidified incubator. Total cells were separated into 3 groups: (1) miR-18a mimic group (cells transfected with miR-18a mimic); (2) miR-18a inhibitor group (cells transfected with miR-18a inhibitor); and (3) control group. Transfection efficiency was monitored by RT-qPCR. miR-18a mimic and miR-18a inhibitor were synthesized by Genepharma (Shanghai, China). Cells between passage 2 and passage 5 were used in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!