MPTP hydrochloride (MPTP · HCl) (M0896) and MPP+ iodide (MPP+· I) (D048) were obtained from Sigma Chemical Company (St. Louis, MO). Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12), penicillin, streptomycin, and fetal bovine serum (FBS) were from Gibco (Grand Island, NY). Mouse anti-EEA1 (ab70521) and mouse anti-mitochondrial (MTC02) (ab3298) antibodies were purchased from Abcam (New Territories, Hong Kong). Mouse anti-calnexin (sc-46669), rabbit anti-Sg II (sc-50290), and Sg III (sc-50289) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-golgin-97 (human)-M (A21270), Alexa Fluor 488 donkey anti-mouse IgG, and Alexa Fluor 594 donkey anti-rabbit IgG (ab150076)-conjugated secondary antibodies were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The MTS CellTiter 96®Aqueous One Solution Reagent was from Promega (Madison, WI, USA). RNAiso Plus and SYBR Prime EX Taq were from TaKaRa Biotechnology (Shiga, Japan). Evo M-MLV RT Premix was from Accurate Biotechnology (Hunan, China). The BCA Protein Assay Kit and Easysee reagent were from Beyotime Biotechnology (Beijing, China) and TransGen Biotech (Beijing, China).
Sybr prime ex taq
Manufactured by Takara Bio
Sourced in Japan
SYBR Prime EX Taq is a high-performance DNA polymerase designed for real-time PCR applications. It is optimized for fast and sensitive detection of target DNA sequences.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rnaiso plus, by Takara Bio (2 mentions)
Bca protein assay kit, by Beyotime (2 mentions)
Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium f12 dmem f12, by Thermo Fisher Scientific (1 mentions)
Celltiter 96 aqueous one solution mts reagent, by Promega (1 mentions)
Evo m mlv rt premix, by Accurate Biology (1 mentions)
Ebioscience annexin 5 fitc apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Cell cycle detection kit, by Thermo Fisher Scientific (1 mentions)
β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Celltiter 96 aqueous one solution reagent, by Promega (1 mentions)
2 protocols using sybr prime ex taq
1
MPTP Neurotoxicity Assay Protocol
2
Neuroprotective Effects of Manganese on Dopaminergic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Dopamine (DA), Manganese(II) chloride tetrahydrate (MnCl 2 •4H 2 O), MPP+ iodide (MPP + is active metabolite of MPTP), and LPS were purchased from Sigma-Aldrich (St. Louis, MO, USA). CellTiter 96 ® Aqueous One Solution Reagent was purchased from Promega (Promega, WI, USA), and the eBioscience™ Annexin V-FITC Apoptosis Detection Kit and Cell Cycle Detection Kit were obtained from Invitrogen (Thermo Fisher, Wa l t h a m , M A , U S A ) a n d K e y g e n ( K e y g e n Biotechnology, Jiangsu, China), respectively. The radioimmunoprecipitation assay (RIPA) reagent and BCA Protein Assay Kit were obtained from Beyotime Biotechnology (Beijing, China). The Easysee reagent was purchased from TransGen Biotech (Beijing, China). Antibodies against SCG3 (SC-50289), SCG2 (SC-50290), and β-actin (SC-47778) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). RNAiso Plus, PrimeScript RT Reagent Kit, and SYBR Prime EX Taq were purchased from Takara Biotechnology (Shiga, Japan).
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