Berberine hydrochloride (BER), 5-Fluorouracil (5-FU) camptothecin (CPT), and Paclitaxel (TAX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). WST-1 cell proliferation and cytotoxicity assay kit, PD98059, and LY294002 were purchased from Beyotime (Haimen, Jiangsu, China). RPMI 1640 medium, Dulbecco's modified Eagle's medium (DMEM), penicillin-streptomycin, trypsin, fetal bovine serum (FBS), and phosphate buffered saline (PBS) were obtained from Gibco (Carlsbad, CA, USA). Antibodies against phosphor-ERK, ERK, phosphor-AKT, AKT and β-actin were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).
Paclitaxel tax
Manufactured by Merck Group
Sourced in United States
Paclitaxel (TAX) is a laboratory reagent used in research applications. It is a microtubule-stabilizing agent that inhibits cell division. Paclitaxel is commonly used in cell biology and cancer research studies.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Phosphor akt, by Cell Signaling Technology (1 mentions)
Ly294002, by Beyotime (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Wst 1 cell proliferation and cytotoxicity assay kit, by Beyotime (1 mentions)
Pd98059, by Beyotime (1 mentions)
Mtt kit, by Merck Group (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Mannitol, by Merck Group (1 mentions)
Soybean phosphatidylcholine spc, by Merck Group (1 mentions)
Xenolight dir, by PerkinElmer (1 mentions)
4 protocols using paclitaxel tax
1
Cytotoxicity Assay of Chemotherapeutics
2
Evaluating Cytotoxicity and Cell Cycle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was evaluated by the MTT kit (Sigma-Aldrich Merck, Darmstadt, Germany), as reported in the relative guidelines. Results were reported as percentage variation compared to the control (CNT), which was considered as a unit (100%). Plant extract cytotoxicity was measured using the Trypan Blue (1%, w/v) exclusion test and counting dead cells by a Neubauer-modified chamber. The selectivity index (SI) of HCOE on tumor and non-tumor cells was measured according to the following formula: SI = IC50 non-tumor cell line/IC50 cancer cell line (considering that IC50 represented the concentration at which 50% of cell proliferation was inhibited). Cell cycle analysis was performed by a FACSCalibur instrument (Beckton and Dickinson, Le Pont-de-Claix, France) associated to CellQuest software, counting 10,000 events per sample and using the protocol documented in Gismondi et al. [40 (link)]. Cytofluorimetric data were shown as a percentage of cells in G0/G1, S, G2/M, and sub-G1 phase. Other treatments were performed by necrostatin-1 (NEC-1; 20 μM, 48 h), Z-VAD-FMK (Z-VAD; 20 μM, 48 h), and Paclitaxel (TAX; 20 nM, 8 h) (Sigma-Aldrich).
3
Somatostatin Analog Synthesis and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paclitaxel (TAX) and Tween-80 were purchased from Sigma (St. Louis, MO, USA). Egg phosphatidylcholine (Egg PC), Cholesterol, and DSPE-PEG2000-azide (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[azido (polyethylene glycol)-2000]) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). To create a somatostatin analog, octreotide (H-D-Phe-Cys*-Phe-D-Trp-Lys-Thr-Cys*-Thr-ol) 5-pentynecarboxylic acid was used to replace the N-terminus of the D-Phenylalanine group with an alkyne group. The resulting 5-pentynecarbonyl-octreotide was synthesized by Peptides International, Inc. (Louisville, KY, USA). Fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagents and dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA). Horse serum was obtained from Thermo Fisher Scientific (Waltham, MA, USA). SSTR2 primers were purchased from Invitrogen (Waltham, MA, USA). Anti-SSTR2 primary antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Horseradish peroxidase (HRP)-conjugated secondary antibody was purchased from Cell Signaling Technology (Danvers, MA, USA).
4
LHRH-Targeted Lipid Nanoparticles for Cancer Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Precirol ATO 5 was generously provided by Gattefossé USA (Paramus, NJ). Soybean phosphatidylcholine (SPC), Paclitaxel (TAX), Squalene, Tween-80, Mannitol were purchased from Sigma Aldrich (St. Louis, MO). DSPE-PEG (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] were obtained from Avanti Polar Lipids (Alabaster, AL). XenoLight DiR obtained from Perkin Elmer (Akron, OH). A modified synthetic analog of Luteinizing Hormone-Releasing Hormone (LHRH) decapeptide (Gln-His-Trp-Ser-Tyr-DLys(D-Cys)-Leu-Arg-Pro) was synthesized according to our design by American Peptide Company, Inc. (Sunnyvale, CA). The sequence of native LHRH peptide, which is similar in human, mouse, and rat, was modified to provide a reactive amino group only on the side chain of a lysine residue, which replaced Gly at the position 6 to yield the superactive, degradation-resistant-Lys-6-des-Gly-10-Pro-9-ethylamide LHRH analog 10 (link)-13 (link). The synthesized sequence of LHRH peptide is highly efficient for targeting of drug delivery systems specifically to the cancer tumors 9 (link), 10 (link), 14 (link)-17 (link) and Cys residue do not influence the recognition process. The remmining materials were obtained from Sigma Aldrich (St. Louis, MO).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!